World-2DPAGE Home
Home  |  Contact

Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P00894




SWISS-2DPAGE:  P00894


P00894


General information about the entry
View entry in simple text format
Entry nameILVH_ECOLI
Primary accession numberP00894
integrated into SWISS-2DPAGE on December 1, 2000 (release 13)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 8)
Name and origin of the protein
DescriptionRecName: Full=Acetolactate synthase isozyme 3 small subunit; EC=2.2.1.6; AltName: Full=ALS-III; AltName: Full=Acetohydroxy-acid synthase III small subunit; Short=AHAS-III;.
Gene nameName=ilvH
Synonyms=brnP
OrderedLocusNames=b0078, JW0077
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI6-11 {Escherichia coli(6-11)}
Escherichia coli
ECOLI6-11
  map experimental info
  protein estimated location
 
ECOLI6-11

MAP LOCATIONS:
pI=8.11; Mw=15587  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP00894; ILVH_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameILVH_ECOLI
Primary accession numberP00894
Secondary accession number(s) P78046 Q47637
Sequence was last modified on July 19, 2003 (version 3)
Annotations were last modified on October 19, 2011 (version 107)
Name and origin of the protein
DescriptionRecName: Full=Acetolactate synthase isozyme 3 small subunit; EC=2.2.1.6; AltName: Full=ALS-III; AltName: Full=Acetohydroxy-acid synthase III small subunit; Short=AHAS-III;
Gene nameName=ilvH
Synonyms=brnP
OrderedLocusNames=b0078, JW0077
Encoded onName=ilvH; Synonyms=brnP; OrderedLocusNames=b0078, JW0077
Keywords3D-structure; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX01609; CAA25756.1; -; Genomic_DNA
EMBLX55457; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLX55034; CAA38855.1; -; Genomic_DNA
EMBLU00096; AAC73189.1; -; Genomic_DNA
EMBLAP009048; BAB96647.2; -; Genomic_DNA
EMBLM35034; AAA24627.1; -; Genomic_DNA
PIRF64729; YCEC3H; .
RefSeqNP_414620.1; NC_000913.2; .
PDB2F1F; X-ray; 1.75 A; A/B=2-163
PDBsum2F1F; -; .
ProteinModelPortalP00894; -; .
SMRP00894; 2-163; .
DIPDIP-10024N; -; .
SWISS-2DPAGEP00894; -; .
EnsemblBacteriaEBESCT00000002562; EBESCP00000002562; EBESCG00000002091; .
EnsemblBacteriaEBESCT00000002563; EBESCP00000002563; EBESCG00000002091; .
EnsemblBacteriaEBESCT00000002564; EBESCP00000002564; EBESCG00000002091; .
EnsemblBacteriaEBESCT00000002565; EBESCP00000002565; EBESCG00000002091; .
EnsemblBacteriaEBESCT00000015938; EBESCP00000015229; EBESCG00000014998; .
GeneID947267; -; .
GenomeReviewsAP009048_GR; JW0077; .
GenomeReviewsU00096_GR; b0078; .
KEGGecj:JW0077; -; .
KEGGeco:b0078; -; .
EchoBASEEB0494; -; .
EcoGeneEG10499; ilvH; .
eggNOGCOG0440; -; .
GeneTreeEBGT00050000011172; -; .
HOGENOMHBG335310; -; .
OMAIERELAM; -; .
ProtClustDBPRK11895; -; .
BioCycEcoCyc:ACETOLACTSYNIII-HCHAIN-MONOMER; -; .
BioCycMetaCyc:ACETOLACTSYNIII-HCHAIN-MONOMER; -; .
GenevestigatorP00894; -; .
GOGO:0003984; F:acetolactate synthase activity; IEA:EC; .
GOGO:0016597; F:amino acid binding; IEA:InterPro; .
GOGO:0009082; P:branched chain family amino acid biosynthetic process; IEA:UniProtKB-KW; .
InterProIPR004789; Acetalactate_synth_ssu; .
InterProIPR019455; Acetolactate_synth_ssu_C; .
InterProIPR002912; ACT-bd; .
PfamPF01842; ACT; 1; .
PfamPF10369; ALS_ss_C; 1; .
TIGRFAMsTIGR00119; Acolac_sm; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server