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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P00963 |
SWISS-2DPAGE: P00963
P00963:
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ID ASNA_ECOLI; STANDARD; 2DG.
AC P00963;
DT 15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT 15-MAY-2003, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 7.
DE RecName: Full=Aspartate--ammonia ligase; EC=6.3.1.1; AltName:
DE Full=Asparagine synthetase A;.
GN Name=asnA; OrderedLocusNames=b3744, JW3722;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI-DIGE4.5-6.5.
IM ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: Homodimer
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WNK=5.45/37876;
2D -!- PI/MW: SPOT 2D-001WNR=5.38/37715;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WNK: (R)LGLIEVQAPILSR(V),27-39;
2D (K)VKALPDAQFEVVHSLAK(W),59-75; (K)ALPDAQFEVVHSLAK(W),61-75;
2D (K)STVEAIWAGIK(A),135-145; (K)DLGAVFLVGIGGK(L),192-204.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WNR: (R)LGLIEVQAPILSR(V),27-39;
2D (K)STVEAIWAGIK(A),135-145; (K)DLGAVFLVGIGGK(L),192-204.
2D -!- EXPRESSION: decrease after benzoic acid treatment [1].
2D -!- MAPPING: Tandem mass spectrometry [1].
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DR UniProtKB/Swiss-Prot; P00963; ASNA_ECOLI.
//
The following 2-D map is available for this protein:
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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