Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

P01024:

ID   CO3_HUMAN; STANDARD; 2DG.
AC   P01024;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   01-DEC-2000, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=Complement C3; AltName: Full=C3 and PZP-like
DE   alpha-2-macroglobulin domain-containing protein 1; Contains: RecName:
DE   Full=Complement C3 beta chain; Contains: RecName: Full=Complement C3
DE   alpha chain; Contains: RecName: Full=C3a anaphylatoxin; Contains:
DE   RecName: Full=Complement C3b alpha' chain; Contains: RecName:
DE   Full=Complement C3c alpha' chain fragment 1; Contains: RecName:
DE   Full=Complement C3dg fragment; Contains: RecName: Full=Complement C3g
DE   fragment; Contains: RecName: Full=Complement C3d fragment; Contains:
DE   RecName: Full=Complement C3f fragment; Contains: RecName: Full=Complement
DE   C3c alpha' chain fragment 2; Flags: Precursor;.
GN   Name=C3; Synonyms=CPAMD1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   CSF_HUMAN, HEPG2SP_HUMAN, PLASMA_HUMAN.
IM   CSF_HUMAN, HEPG2SP_HUMAN, PLASMA_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=93092937; PubMed=1459097; [NCBI, Expasy, EBI, Israel, Japan]
RA   Hughes G.J., Frutiger S., Paquet N., Ravier F., Pasquali C., Sanchez
RA   J.-C., James R.W., Tissot J.-D., Bjellqvist B., Hochstrasser D.F.;
RT   ''''''Plasma protein map: an update by microsequencing'';'';'';
RL   Electrophoresis 13(1):707-714(1992).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
CC   -!- SUBUNIT: ALPHA AND BETA CHAIN 
2D   -!- MASTER: CSF_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000BJ7=5.91/104427;
2D   -!-   PI/MW: SPOT 2D-000BJG=5.99/103408;
2D   -!-   PI/MW: SPOT 2D-000BJL=6.14/102400;
2D   -!-   PI/MW: SPOT 2D-000BJP=6.07/102400;
2D   -!-   PI/MW: SPOT 2D-000BJQ=6.21/101899;
2D   -!-   MAPPING: MATCHING WITH THE PLASMA MASTER GEL [2].
2D   -!- MASTER: HEPG2SP_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0008ME=5.69/106396;
2D   -!-   PI/MW: SPOT 2D-0008MG=5.77/104612;
2D   -!-   PI/MW: SPOT 2D-0008MO=5.85/102280;
2D   -!-   PI/MW: SPOT 2D-0008MR=5.92/101705;
2D   -!-   PI/MW: SPOT 2D-0008MW=6.00/100000;
2D   -!-   PI/MW: SPOT 2D-0008N0=6.13/98894;
2D   -!-   PI/MW: SPOT 2D-0008NI=6.26/98528;
2D   -!-   PI/MW: SPOT 2D-0008NL=6.39/97800;
2D   -!-   MAPPING: MATCHING WITH THE PLASMA MASTER GEL [2].
2D   -!- MASTER: PLASMA_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0004JD=6.81/70675;
2D   -!-   PI/MW: SPOT 2D-0004JH=6.50/71216;
2D   -!-   PI/MW: SPOT 2D-0004JI=6.66/70675;
2D   -!-   PI/MW: SPOT 2D-0004JM=6.41/71488;
2D   -!-   PI/MW: SPOT 2D-0004K0=6.98/70675;
2D   -!-   PI/MW: SPOT 2D-00052J=4.84/40915;
2D   -!-   NORMAL LEVEL: 550-1200 MG/L.
2D   -!-   PATHOLOGICAL LEVEL: DECREASED DURING LUPUS ERYTHEMATOSUS AND
2D         MEMBRANOPROLIFERATIVE GLOMERULONEPHRITIS; INCREASED DURING ACUTE
2D         INFLAMMATORY REACTIONS.
2D   -!-   MAPPING: MICROSEQUENCING [1].
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DR   DOSAC-COBS 2D-PAGE; P01024; P01024.
DR   DOSAC-COBS-2DPAGE; P01024; CO3_HUMAN.
DR   Siena-2DPAGE; P01024; CO3_HUMAN.
DR   UniProtKB/Swiss-Prot; P01024; CO3_HUMAN.
//

The following 2-D maps are available for this protein:

	Cerebrospinal Fluid	HepG2 Secreted Proteins	Plasma

SWISS-2DPAGE (search AC)