|
|
|
|
|
Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P02358 |
SWISS-2DPAGE: P02358
P02358:
Nice View - a user-friendly view of this entry
ID RS6_ECOLI; STANDARD; 2DG.
AC P02358;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 12.
DE RecName: Full=30S ribosomal protein S6; Contains: RecName: Full=30S
DE ribosomal protein S6, fully modified isoform; Contains: RecName: Full=30S
DE ribosomal protein S6, non-modified isoform;.
GN Name=rpsF; OrderedLocusNames=b4200, JW4158;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
RA Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT ''''''Protein identifications of O'Farrell two-dimensional gels:
RT locations of 81 Escherichia coli proteins'';'';'';
RL J. Bacteriol. 141(1):1409-1420(1980).
RN [4]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [5]
RP PROTEIN EXPRESSION.
RX MEDLINE=78212912; PubMed=352533; [NCBI, Expasy, EBI, Israel, Japan]
RA Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.;
RT ''''''Patterns of protein synthesis in E. coli: a catalog of the amount
RT of 140 individual proteins at different growth rates'';'';'';
RL Cell 14(1):179-190(1978).
RN [6]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000LQ6=5.31/15845;
2D -!- PI/MW: SPOT 2D-000LQ7=5.15/15865;
2D -!- PI/MW: SPOT 2D-000LQA=5.25/15845;
2D -!- PI/MW: SPOT 2D-000LUR=5.65/11023;
2D -!- EXPRESSION: INCREASED IN LEVEL WITH INCREASING GROWTH RATE [5].
2D -!- MAPPING: SPOTS 2D-000LQ6, 2D-000LQ7, 2D-000LQA: GEL MATCHING OR
2D AMINO ACID COMPOSITION AND/OR MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED
2D ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3]; SPOT 2D-000LUR: AMINO ACID
2D COMPOSITION AND SEQUENCE TAG (MRHY) [4].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WXA=5.30/16076;
2D -!- PI/MW: SPOT 2D-001WXJ=5.20/16000;
2D -!- PEPTIDE MASSES: SPOT 2D-001WXA: 931.43; 946.46; 1081.47; 1102.6;
2D 1181.56; 1398.68; 1487.78; 2056.81; 2704.4; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WXJ: 931.39; 946.46; 1081.48; 1181.55;
2D 1324.69; 1487.75; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WXA: (R)YTAAITGAEGK(I),25-35;
2D (R)RQLAYPINK(L),45-53; (R)QLAYPINK(L),46-53.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WXJ: (R)YTAAITGAEGK(I),25-35; (R)QLAYPINK(L),46-53.
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [6].
CC ---------------------------------------------------------------------------
CC This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC There are no restrictions on its use by non-profit institutions as long as
CC its content is in no way modified and this statement is not removed.
CC Usage by and for commercial entities requires a license agreement (See
CC http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC ---------------------------------------------------------------------------
DR ECO2DBASE; D014.7; 6TH EDITION.
DR ECO2DBASE; C014.8; 6TH EDITION.
DR ECO2DBASE; C015.3; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P02358; RS6_ECOLI.
//
The following 2-D maps are available for this protein:
|
Escherichia coli
 |
Escherichia coli DIGE (4.5-6.5)
 |
|---|
SWISS-2DPAGE (search AC)
|
