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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P02358




SWISS-2DPAGE: P02358



P02358:

Nice View - a user-friendly view of this entry
ID   RS6_ECOLI; STANDARD; 2DG.
AC   P02358;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=30S ribosomal protein S6; Contains: RecName: Full=30S
DE   ribosomal protein S6, fully modified isoform; Contains: RecName: Full=30S
DE   ribosomal protein S6, non-modified isoform;.
GN   Name=rpsF; OrderedLocusNames=b4200, JW4158;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT   ''''''Protein identifications of O'Farrell two-dimensional gels:
RT   locations of 81 Escherichia coli proteins'';'';'';
RL   J. Bacteriol. 141(1):1409-1420(1980).
RN   [4]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [5]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=78212912; PubMed=352533; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.;
RT   ''''''Patterns of protein synthesis in E. coli: a catalog of the amount
RT   of 140 individual proteins at different growth rates'';'';'';
RL   Cell 14(1):179-190(1978).
RN   [6]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000LQ6=5.31/15845;
2D   -!-   PI/MW: SPOT 2D-000LQ7=5.15/15865;
2D   -!-   PI/MW: SPOT 2D-000LQA=5.25/15845;
2D   -!-   PI/MW: SPOT 2D-000LUR=5.65/11023;
2D   -!-   EXPRESSION: INCREASED IN LEVEL WITH INCREASING GROWTH RATE [5].
2D   -!-   MAPPING: SPOTS 2D-000LQ6, 2D-000LQ7, 2D-000LQA: GEL MATCHING OR
2D         AMINO ACID COMPOSITION AND/OR MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED
2D         ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3]; SPOT 2D-000LUR: AMINO ACID
2D         COMPOSITION AND SEQUENCE TAG (MRHY) [4].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WXA=5.30/16076;
2D   -!-   PI/MW: SPOT 2D-001WXJ=5.20/16000;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WXA: 931.43; 946.46; 1081.47; 1102.6;
2D         1181.56; 1398.68; 1487.78; 2056.81; 2704.4; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WXJ: 931.39; 946.46; 1081.48; 1181.55;
2D         1324.69; 1487.75; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WXA: (R)YTAAITGAEGK(I),25-35;
2D         (R)RQLAYPINK(L),45-53; (R)QLAYPINK(L),46-53.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WXJ: (R)YTAAITGAEGK(I),25-35; (R)QLAYPINK(L),46-53.
2D   -!-   MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [6].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; D014.7; 6TH EDITION.
DR   ECO2DBASE; C014.8; 6TH EDITION.
DR   ECO2DBASE; C015.3; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P02358; RS6_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server