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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P02545 |
SWISS-2DPAGE: P02545
P02545:
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ID LMNA_HUMAN; STANDARD; 2DG.
AC P02545; P02546;
DT 01-APR-2000, integrated into SWISS-2DPAGE (release 12).
DT 15-MAY-2003, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 9.
DE RecName: Full=Prelamin-A/C; Contains: RecName: Full=Lamin-A/C; AltName:
DE Full=70 kDa lamin; AltName: Full=Renal carcinoma antigen NY-REN-32;
DE Flags: Precursor;.
GN Name=LMNA; Synonyms=LMN1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Hominidae; Homo.
OX NCBI_TaxID=9606;
MT NUCLEI_LIVER_HUMAN, NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
IM NUCLEI_LIVER_HUMAN, NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=20529951; PubMed=11079567; [NCBI, Expasy, EBI, Israel, Japan]
RA Jung E., Hoogland C., Chiappe D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''The establishment of a human liver nuclei 2-DE reference map'';'';'';
RL Electrophoresis 21(1):3483-3487(2000).
RN [2]
RP MAPPING ON GEL.
RX PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA Greco A., Hochstrasser D.F., Diaz J.-J.;
RT ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL Mol. Biol. Cell. 13(1):4100-4109(2002).
1D -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D -!- MW: BAND 1D-001V8C=63488;
1D -!- MAPPING: Peptide mass fingerprinting [2].
2D -!- MASTER: NUCLEI_LIVER_HUMAN;
2D -!- PI/MW: SPOT 2D-001IZK=7.15/68933;
2D -!- PI/MW: SPOT 2D-001IZM=7.00/68933;
2D -!- PI/MW: SPOT 2D-001IZR=7.11/68566;
2D -!- PI/MW: SPOT 2D-001J2X=7.11/59687;
2D -!- PI/MW: SPOT 2D-001J35=6.91/59370;
2D -!- PI/MW: SPOT 2D-001J37=7.04/59370;
2D -!- PEPTIDE MASSES: SPOT 2D-001IZK: 807.396; 849.447; 859.431; 918.429;
2D 919.43; 972.503; 974.477; 1023.511; 1028.583; 1043.551; 1057.646;
2D 1089.557; 1102.544; 1131.522; 1165.552; 1171.669; 1182.62; 1291.626;
2D 1331.687; 1359.698; 1363.617; 1566.747; 1752.858; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001IZM: 849.446; 918.434; 919.443; 972.508;
2D 1023.523; 1028.582; 1043.567; 1089.55; 1102.583; 1131.556; 1165.575;
2D 1171.646; 1182.641; 1291.631; 1331.702; 1347.711; 1359.713; 1363.67;
2D 1502.77; 1566.805; 1752.898; 2365.192; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001IZR: 849.458; 919.444; 972.497; 974.483;
2D 1023.514; 1028.577; 1043.549; 1057.638; 1089.57; 1102.595; 1131.557;
2D 1148.588; 1165.582; 1171.657; 1176.636; 1182.628; 1291.639; 1331.708;
2D 1347.707; 1359.701; 1363.67; 1430.835; 1459.774; 1491.79; 1566.79;
2D 1752.93; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001J2X: 806.924; 848.998; 918.003; 949.053;
2D 972.078; 1023.099; 1028.153; 1089.16; 1102.172; 1131.166; 1148.218;
2D 1165.212; 1171.284; 1182.26; 1291.301; 1331.419; 1344.385; 1347.366;
2D 1359.396; 1363.356; 1430.528; 1491.48; 1502.477; 1752.698; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001J35: 919.417; 1023.506; 1028.56;
2D 1089.566; 1165.57; 1182.601; 1291.623; 1359.69; 1752.855; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001J37: 807.396; 849.461; 918.429; 919.442;
2D 972.528; 974.487; 1023.504; 1028.565; 1043.535; 1089.552; 1131.531;
2D 1148.581; 1165.581; 1182.637; 1291.635; 1359.709; 1363.634; 1491.733;
2D 1752.87; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D -!- PI/MW: SPOT 2D-001VG3=6.76/75283;
2D -!- PI/MW: SPOT 2D-001VI5=6.68/64287;
2D -!- PEPTIDE MASSES: SPOT 2D-001VG3: 919.3; 1028.5; 1089.5; 1104.5;
2D 1171.6; 1187.5; 1243.6; 1358.7; 1381.6; 1417.7; 1447.7; 1491.7; 1502.7;
2D 1507.7; 1566.7; 1605.8; 1619.8; 1629.8; 1635.8; 1665.8; 1699.9; 1752.8;
2D 1761.9; 1961.9; 1972.9; 2324.1; 2365.1; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001VI5: 919.4; 1028.6; 1089.6; 1165.5;
2D 1171.7; 1187.6; 1243.7; 1275.7; 1304.6; 1358.8; 1359.7; 1381.7; 1417.8;
2D 1507.8; 1605.9; 1619.9; 1629.9; 1635.9; 1649.8; 1676.9; 1688; 1700.1;
2D 1730; 1753; 1962.1; 2324.3; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [2].
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DR REPRODUCTION-2DPAGE; P02545; P02545.
DR REPRODUCTION-2DPAGE; IPI00216952; IPI00216952.
DR REPRODUCTION-2DPAGE; IPI00021405; IPI00021405.
DR Siena-2DPAGE; P02545; LMNA_HUMAN.
DR UniProtKB/Swiss-Prot; P02545; LMNA_HUMAN.
//
The following 2-D maps are available for this protein:
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Soluble nuclear proteins and matrix from liver tissue
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SDS-PAGE of nucleolar proteins from Human HeLa cells
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2D-PAGE of nucleolar proteins from Human HeLa cells
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SWISS-2DPAGE (search AC)
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