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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P04816 |
SWISS-2DPAGE: P04816
P04816
| General information about the entry |
| View entry in simple text format |
| Entry name | LIVK_ECOLI |
| Primary accession number | P04816 |
| integrated into SWISS-2DPAGE on | August 1, 1995 (release 2) |
| 2D Annotations were last modified on | May 15, 2003 (version 4) |
| General Annotations were last modified on | May 19, 2011 (version 13) |
| Name and origin of the protein |
| Description | RecName: Full=Leucine-specific-binding protein; Short=L-BP; Short=LS-BP; Flags: Precursor;. |
| Gene name | Name=livK
OrderedLocusNames=b3458, JW3423
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| Annotated species | Escherichia coli [TaxID: 562] |
| Taxonomy | Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. |
| References |
| [1] |
MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
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| [2] |
MAPPING ON GEL
MEDLINE=83270974; PubMed=6308410; [NCBI, Expasy, EBI, Israel, Japan]
Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.
''''''Gene-protein index of Escherichia coli K-12'';'';''
Microbiol. Rev. 47(1):231-284(1983)
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| [3] |
MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
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| [4] |
PROTEIN EXPRESSION
MEDLINE=94042879; PubMed=8226654; [NCBI, Expasy, EBI, Israel, Japan]
Gage D.J., Neidhardt F.C.
''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';''
J. Bacteriol. 175(1):7105-7108(1993)
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| [5] |
PROTEIN EXPRESSION
MEDLINE=93352420; PubMed=8349554; [NCBI, Expasy, EBI, Israel, Japan]
Sankar P., Hutton M.E., Vanbogelen R.A., Clark R.L., Neidhardt F.C.
''''''Expression analysis of cloned chromosomal segments of Escherichia coli'';'';''
J. Bacteriol. 175(1):5145-5152(1993)
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| [6] |
MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
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| [7] |
MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
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| | 2D PAGE maps for identified proteins
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How to interpret a protein
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ECOLI {Escherichia coli}
Escherichia coli
map experimental info
protein estimated location
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ECOLI
MAP LOCATIONS:
EXPRESSION:
INCREASED FOLLOWING EXPOSURE TO DNP [4]; WAS ENCODED AND EXPRESSED FROM PLASMID PFNK613L [5].
MAPPING (identification):
AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT [2] AND VANBOGELEN [3].
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ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
map experimental info
protein estimated location
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ECOLI4.5-5.5
MAP LOCATIONS:
MAPPING (identification):
Peptide mass fingerprinting [6];
SPOT 2D-00152C: MICROSEQUENCING (DDIK) [6]. |
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ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
map experimental info
protein estimated location
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ECOLI5-6
MAP LOCATIONS:
MAPPING (identification):
Peptide mass fingerprinting [6].
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ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
map experimental info
protein estimated location
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ECOLI-DIGE4.5-6.5
MAP LOCATIONS:
EXPRESSION:
decrease after benzoic acid treatment [7].
MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [7].
| | Copyright |
| This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). |
| Cross-references |
| 2DBase-Ecoli | P04816; LIVK_ECOLI. |
| ECO2DBASE | B040.8; 6TH EDITION. |
| UniProtKB/Swiss-Prot | P04816; LIVK_ECOLI. |
| 2D PAGE maps for identified proteins
|
- How to interpret a protein map
- You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
- Warning 1: the displayed region reflects
an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information.
It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
- Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
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SWISS-2DPAGE (search AC)
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