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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P04816 |
SWISS-2DPAGE: P04816
P04816:
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ID LIVK_ECOLI; STANDARD; 2DG.
AC P04816;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 13.
DE RecName: Full=Leucine-specific-binding protein; Short=L-BP; Short=LS-BP;
DE Flags: Precursor;.
GN Name=livK; OrderedLocusNames=b3458, JW3423;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RX MEDLINE=83270974; PubMed=6308410; [NCBI, Expasy, EBI, Israel, Japan]
RA Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.;
RT ''''''Gene-protein index of Escherichia coli K-12'';'';'';
RL Microbiol. Rev. 47(1):231-284(1983).
RN [3]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [4]
RP PROTEIN EXPRESSION.
RX MEDLINE=94042879; PubMed=8226654; [NCBI, Expasy, EBI, Israel, Japan]
RA Gage D.J., Neidhardt F.C.;
RT ''''''Adaptation of Escherichia coli to the uncoupler of oxidative
RT phosphorylation 2,4-dinitrophenol'';'';'';
RL J. Bacteriol. 175(1):7105-7108(1993).
RN [5]
RP PROTEIN EXPRESSION.
RX MEDLINE=93352420; PubMed=8349554; [NCBI, Expasy, EBI, Israel, Japan]
RA Sankar P., Hutton M.E., Vanbogelen R.A., Clark R.L., Neidhardt F.C.;
RT ''''''Expression analysis of cloned chromosomal segments of Escherichia coli'';'';'';
RL J. Bacteriol. 175(1):5145-5152(1993).
RN [6]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [7]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KZS=5.00/41546;
2D -!- EXPRESSION: INCREASED FOLLOWING EXPOSURE TO DNP [4]; WAS ENCODED
2D AND EXPRESSED FROM PLASMID PFNK613L [5].
2D -!- MAPPING: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND
2D IDENTIFIED ON 2-D GELS BY NEIDHARDT [2] AND VANBOGELEN [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-00152C=5.08/39816;
2D -!- PI/MW: SPOT 2D-00158X=5.56/29053;
2D -!- PEPTIDE MASSES: SPOT 2D-00152C: 920.466; 1021.548; 1246.774;
2D 1723.957; 1802.997; 2028.039; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-00158X: 920.43; 1021.51; 1246.74; 1803.1;
2D 1812; 2597.43; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [6]; SPOT 2D-00152C:
2D MICROSEQUENCING (DDIK) [6].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001KXJ=5.25/59333;
2D -!- PEPTIDE MASSES: SPOT 2D-001KXJ: 892.485; 920.424; 1021.504;
2D 1129.594; 1246.708; 1480.637; 1802.873; 2027.89; 2112.009; 2445.033;
2D 2461.004; 2508.195; 2597.261; 2636.287; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [6].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WMV=5.09/39439;
2D -!- PEPTIDE MASSES: SPOT 2D-001WMV: 892.43; 1021.42; 1129.47; 1246.61;
2D 1303.58; 1480.59; 1802.74; 1811.8; 2027.78; 2507.94; 2597.02; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WMV: (R)TAGLDSSQGPTAAK(Y),140-153;
2D (K)AANANVVFFDGITAGEKDFSALIAR(L),187-211; (R)TGSDEPLALVK(D),317-327.
2D -!- EXPRESSION: decrease after benzoic acid treatment [7].
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [7].
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DR 2DBase-Ecoli; P04816; LIVK_ECOLI.
DR ECO2DBASE; B040.8; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P04816; LIVK_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4.5-5.5)
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Escherichia coli(5-6)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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