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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P05055




SWISS-2DPAGE: P05055



P05055:

Nice View - a user-friendly view of this entry
ID   PNP_ECOLI; STANDARD; 2DG.
AC   P05055;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 15.
DE   RecName: Full=Polyribonucleotide nucleotidyltransferase; EC=2.7.7.8;
DE   AltName: Full=Polynucleotide phosphorylase; Short=PNPase;.
GN   Name=pnp; OrderedLocusNames=b3164, JW5851;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=83270974; PubMed=6308410; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.;
RT   ''''''Gene-protein index of Escherichia coli K-12'';'';'';
RL   Microbiol. Rev. 47(1):231-284(1983).
RN   [3]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [4]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=87194592; PubMed=3553157; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Jones P.G., Vanbogelen R.A., Neidhardt F.C.;
RT   ''''''Induction of proteins in response to low temperature in Escherichia coli'';'';'';
RL   J. Bacteriol. 169(1):2092-2095(1987).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [6]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: HOMOTRIMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KFR=5.10/83954;
2D   -!-   EXPRESSION: INCREASED DURING THE ADJUSTMENT OF E. COLI TO GROWTH AT
2D         10C [4].
2D   -!-   MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT
2D         [2] AND VANBOGELEN [3].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-0014RF=5.13/87327;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0014RF: 989.492; 991.478; 1488.772;
2D         1514.823; 1677.929; 2010.032; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [5].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WD2=5.16/80468;
2D   -!-   PI/MW: SPOT 2D-001WDK=5.15/78105;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WD2: 935.5; 991.45; 1116.64; 1206.65;
2D         1260.69; 1309.72; 1622.84; 1971.03; 2010.06; 2022.02; 2081.99; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WDK: (K)AKPGQDFFPLTVNYQER(T),50-66;
2D         (R)EGRPSEGETLIAR(L),81-93; (R)YAQVDVIK(S),267-274;
2D         (K)SETIATLLAEDETLDENELGEILHAIEK(N),275-302;
2D         (R)GETQALVTATLGTAR(D),346-360; (R)IVDFGAFVAIGGGK(E),632-645.
2D   -!-   EXPRESSION: decrease after benzoic acid treatment [6].
2D   -!-   MAPPING: SPOT 2D-001WD2: Peptide mass fingerprinting [6]; SPOT
2D         2D-001WDK: Tandem mass spectrometry [6].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; C078.0; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P05055; PNP_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server