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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P05783




SWISS-2DPAGE: P05783



P05783:

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ID   K1C18_HUMAN; STANDARD; 2DG.
AC   P05783;
DT   15-JAN-1999, integrated into SWISS-2DPAGE (release 9).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 9.
DE   RecName: Full=Keratin, type I cytoskeletal 18; AltName: Full=Cell
DE   proliferation-inducing gene 46 protein; AltName: Full=Cytokeratin-18;
DE   Short=CK-18; AltName: Full=Keratin-18; Short=K18;.
GN   Name=KRT18; Synonyms=CYK18; ORFNames=PIG46;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN, NUCLEI_LIVER_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
IM   DLD1_HUMAN, NUCLEI_LIVER_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=20529951; PubMed=11079567; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Jung E., Hoogland C., Chiappe D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''The establishment of a human liver nuclei 2-DE reference map'';'';'';
RL   Electrophoresis 21(1):3483-3487(2000).
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001E4Y=5.25/43007;
2D   -!-   PI/MW: SPOT 2D-001E58=5.15/43228;
2D   -!-   PI/MW: SPOT 2D-001E5D=5.19/43007;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001E4Y: 889.409; 965.432; 975.405; 1041.56;
2D         1065.51; 1267.6; 1292.64; 1319.64; 1419.74; 1522.78; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001E58: 889.491; 965.494; 975.466;
2D         1041.629; 1065.564; 1292.675; 1319.679; 1419.739; 1522.739; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001E5D: 889.514; 965.479; 975.47; 1041.619;
2D         1065.577; 1267.661; 1292.688; 1319.689; 1419.755; 1522.798; 1884.034; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
2D   -!- MASTER: NUCLEI_LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001JD7=5.44/42868;
2D   -!-   PI/MW: SPOT 2D-001JDM=5.34/42868;
2D   -!-   PI/MW: SPOT 2D-001JGE=4.96/39878;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001JD7: 758.432; 807.393; 837.445; 848.382;
2D         924.52; 965.478; 975.46; 982.444; 1004.509; 1012.475; 1028.493; 1041.613;
2D         1065.564; 1093.502; 1109.489; 1174.628; 1239.658; 1267.652; 1286.748;
2D         1292.691; 1319.684; 1395.722; 1419.75; 1506.77; 1522.724; 1883.981; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001JDM: 758.418; 807.392; 837.44; 889.446;
2D         924.527; 965.48; 975.458; 982.441; 1012.485; 1028.49; 1041.617; 1065.566;
2D         1093.509; 1109.504; 1239.663; 1255.646; 1267.658; 1286.717; 1292.692;
2D         1319.688; 1395.735; 1419.763; 1506.77; 1522.766; 1884; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001JGE: 758.417; 807.384; 837.433; 889.434;
2D         924.518; 965.468; 982.443; 1012.484; 1041.607; 1065.557; 1093.512;
2D         1174.619; 1221.639; 1239.645; 1267.649; 1286.701; 1292.733; 1319.685;
2D         1395.726; 1419.767; 1506.766; 1522.785; 1884.05; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001VND=5.59/45837;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001VND: 965.7; 975.7; 1041.9; 1256; 1268;
2D         1293; 1320; 1396.1; 1420.1; 1523.2; 1884.5; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [3].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   PMMA-2DPAGE; P05783; K1CR_HUMAN.
DR   Siena-2DPAGE; P05783; K1C18_HUMAN.
DR   UniProtKB/Swiss-Prot; P05783; K1C18_HUMAN.
//


The following 2-D maps are available for this protein:
Colorectal adenocarcinoma cell line (DL-1)
DLD1_HUMAN
Soluble nuclear proteins and matrix from liver tissue
NUCLEI_LIVER_HUMAN
2D-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_2D_HUMAN


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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server