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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P05787 |
SWISS-2DPAGE: P05787
P05787:
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ID K2C8_HUMAN; STANDARD; 2DG.
AC P05787;
DT 15-JAN-1999, integrated into SWISS-2DPAGE (release 9).
DT 15-MAY-2003, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 9.
DE RecName: Full=Keratin, type II cytoskeletal 8; AltName:
DE Full=Cytokeratin-8; Short=CK-8; AltName: Full=Keratin-8; Short=K8;
DE AltName: Full=Type-II keratin Kb8;.
GN Name=KRT8; Synonyms=CYK8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Hominidae; Homo.
OX NCBI_TaxID=9606;
MT DLD1_HUMAN, NUCLEI_LIVER_HUMAN, NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
IM DLD1_HUMAN, NUCLEI_LIVER_HUMAN, NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
RN [1]
RP MAPPING ON GEL.
RA Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA Appel R.D., Hochstrasser D.F.;
RL Submitted (JAN-1999) to SWISS-2DPAGE.
RN [2]
RP MAPPING ON GEL.
RX MEDLINE=20529951; PubMed=11079567; [NCBI, Expasy, EBI, Israel, Japan]
RA Jung E., Hoogland C., Chiappe D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''The establishment of a human liver nuclei 2-DE reference map'';'';'';
RL Electrophoresis 21(1):3483-3487(2000).
RN [3]
RP MAPPING ON GEL.
RX PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA Greco A., Hochstrasser D.F., Diaz J.-J.;
RT ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL Mol. Biol. Cell. 13(1):4100-4109(2002).
1D -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D -!- MW: BAND 1D-001V8J=53744;
1D -!- PEPTIDE SEQUENCES: BAND 1D-001V8J: (R)LEGLTDEINFLR(Q),213-224; (R)ELQSQISDTSVVLSMoxDNSR(S),233-251.
1D -!- MAPPING: Tandem mass spectrometry [3].
2D -!- MASTER: DLD1_HUMAN;
2D -!- PI/MW: SPOT 2D-001E4E=5.11/43451;
2D -!- PI/MW: SPOT 2D-001E6P=5.04/41700;
2D -!- PEPTIDE MASSES: SPOT 2D-001E4E: 1079.51; 1129.59; 1344.65; 1419.72; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001E6P: 932.471; 1079.52; 1129.61; 1344.67;
2D 1352.7; 1419.73; 1762.99; 1879.85; 2124.99; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: NUCLEI_LIVER_HUMAN;
2D -!- PI/MW: SPOT 2D-001J7V=5.62/50410;
2D -!- PI/MW: SPOT 2D-001J8J=5.74/49414;
2D -!- PEPTIDE MASSES: SPOT 2D-001J7V: 766.353; 827.427; 868.398; 870.42;
2D 906.476; 911.418; 932.46; 979.469; 1030.564; 1045.552; 1066.516;
2D 1079.534; 1129.623; 1137.604; 1153.57; 1169.6; 1208.619; 1277.721;
2D 1320.69; 1344.687; 1352.714; 1405.815; 1419.756; 1792.898; 1847.781;
2D 1863.82; 1879.802; 2003.942; 2109.003; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001J8J: 766.367; 827.427; 870.439; 906.487;
2D 911.422; 932.473; 979.475; 1000.581; 1030.57; 1045.573; 1066.53; 1079.54;
2D 1129.635; 1137.589; 1153.559; 1169.532; 1208.626; 1277.727; 1320.69;
2D 1344.701; 1352.72; 1405.812; 1419.773; 1475.75; 1792.894; 1847.814;
2D 1863.812; 1879.814; 2109.01; 2124.991; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [2].
2D -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D -!- PI/MW: SPOT 2D-001VKW=5.86/53474;
2D -!- PEPTIDE MASSES: SPOT 2D-001VKW: 1277.5; 1320.4; 1336.4; 1344.4;
2D 1368.5; 1419.6; 1428.5; 1813.7; 1863.7; 1879.7; 2034; 2050; 2125; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001VKW: (R)LEGLTDEINFLR(Q),213-224;
2D (R)SLDMoxDSIIAEVK(A),252-263; (R)ASLEAAIADAEQR(G),328-340.
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [3].
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DR Siena-2DPAGE; P05787; K2C8_HUMAN.
DR UniProtKB/Swiss-Prot; P05787; K2C8_HUMAN.
//
The following 2-D maps are available for this protein:
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Colorectal adenocarcinoma cell line (DL-1)
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Soluble nuclear proteins and matrix from liver tissue
 |
SDS-PAGE of nucleolar proteins from Human HeLa cells
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2D-PAGE of nucleolar proteins from Human HeLa cells
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SWISS-2DPAGE (search AC)
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