World-2DPAGE Home
Home  |  Contact
Due to scheduled maintenance work, this service will not be available from Tuesday August 23rd 06:00 pm until Wednesday August 24th 08:00 am CEST. Apologies for the inconvenience.

Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P06748




SWISS-2DPAGE: P06748



P06748:

Nice View - a user-friendly view of this entry
ID   NPM_HUMAN; STANDARD; 2DG.
AC   P06748;
DT   15-JAN-1999, integrated into SWISS-2DPAGE (release 9).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Nucleophosmin; Short=NPM; AltName: Full=Nucleolar
DE   phosphoprotein B23; AltName: Full=Nucleolar protein NO38; AltName: Full=Numatrin;.
GN   Name=NPM1; Synonyms=NPM;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN, NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
IM   DLD1_HUMAN, NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V8Y=41199;
1D   -!-   MW: BAND 1D-001V8Z=40297;
1D   -!-   MW: BAND 1D-001V90=39648;
1D   -!-   MW: BAND 1D-001V91=38781;
1D   -!-   MW: BAND 1D-001V92=38269;
1D   -!-   MW: BAND 1D-001V93=37709;
1D   -!-   MW: BAND 1D-001V94=37156;
1D   -!-   MW: BAND 1D-001V96=35286;
1D   -!-   MW: BAND 1D-001V9I=30626;
1D   -!-   MW: BAND 1D-001V9W=24176; !FRAGMENT!
1D   -!-   MW: BAND 1D-001VA0=22406; !FRAGMENT!
1D   -!-   MW: BAND 1D-001VA5=20822; !FRAGMENT!
1D   -!-   MW: BAND 1D-001VA6=20347; !FRAGMENT!
1D   -!-   PEPTIDE MASSES: BAND 1D-001V91: 910.5; 931.5; 974.5; 1023.5;
1D         1568.7; 1819.8; 1835.8; 2145; 2161; 2227.2; 2243.2; 2929.5; 2945.5; TRYPSIN.
1D   -!-   PEPTIDE MASSES: BAND 1D-001V93: 931.5; 974.5; 1023.5; 1568.7; 2161;
1D         2227.2; 2243.2; 2929.5; 2945.5; TRYPSIN.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8Y:
1D         (R)TVSLGAGAKDELHIVEAEAMoxNYEGSPIK(V),46-73;
1D         (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101; (K)MSVQPTVSLGGFEITPPVVLR(L),81-101; (R)MoxTDQEAIQDLWQWR(K),278-291.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8Z:
1D         (R)TVSLGAGAKDELHIVEAEAMoxNYEGSPIK(V),46-73;
1D         (K)DELHIVEAEAMNYEGSPIK(V),55-73; (K)DELHIVEAEAMoxNYEGSPIK(V),55-73;
1D         (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101; (K)MSVQPTVSLGGFEITPPVVLR(L),81-101;
1D         (K)LAADEDDDDDDEEDDDEDDDDDDFDDEEAEEK(A),158-189; (R)MoxTDQEAIQDLWQWR(K),278-291.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V90:
1D         (R)TVSLGAGAKDELHIVEAEAMNYEGSPIK(V),46-73;
1D         (R)TVSLGAGAKDELHIVEAEAMoxNYEGSPIK(V),46-73;
1D         (K)DELHIVEAEAMNYEGSPIK(V),55-73; (K)DELHIVEAEAMoxNYEGSPIK(V),55-73;
1D         (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101; (K)MSVQPTVSLGGFEITPPVVLR(L),81-101;
1D         (K)LAADEDDDDDDEEDDDEDDDDDDFDDEEAEEK(A),158-189; (R)MoxTDQEAIQDLWQWR(K),278-291.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V91: (K)ADKDYHFK(V),25-32;
1D         (K)VDNDENEHQLSLR(T),33-45; (K)DELHIVEAEAMNYEGSPIK(V),55-73;
1D         (K)DELHIVEAEAMoxNYEGSPIK(V),55-73; (K)SNQNGKDSKPSSTPR(S),207-221;
1D         (R)SKGQESFK(K),222-229; (K)GPSSVEDIK(A),240-248; (K)MoxQASIEK(G),251-257;
1D         (R)MoxTDQEAIQDLWQWR(K),278-291; (R)MTDQEAIQDLWQWR(K),278-291.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V92: (K)VDNDENEHQLSLR(T),33-45;
1D         (R)TVSLGAGAKDELHIVEAEAMNYEGSPIK(V),46-73;
1D         (R)TVSLGAGAKDELHIVEAEAMoxNYEGSPIK(V),46-73;
1D         (K)DELHIVEAEAMNYEGSPIK(V),55-73; (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101;
1D         (K)MSVQPTVSLGGFEITPPVVLR(L),81-101;
1D         (K)LAADEDDDDDDEEDDDEDDDDDDFDDEEAEEK(A),158-189;
1D         (R)MoxTDQEAIQDLWQWR(K),278-291; (R)MTDQEAIQDLWQWR(K),278-291.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V93: (K)VDNDENEHQLSLR(T),33-45; (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V94: (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V96: (K)VDNDENEHQLSLR(T),33-45; (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V9I: (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V9W: (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001VA0: (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001VA5:
1D         (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101; (K)MSVQPTVSLGGFEITPPVVLR(L),81-101.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001VA6: (K)VDNDENEHQLSLR(T),33-45;
1D         (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101; (K)GQESFKK(Q),224-230.
1D   -!-   MAPPING: Tandem mass spectrometry [2]; BANDS 1V91, 1V93: PEPTIDE
1D         MASS FINGERPRINTING [2].
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001EDQ=4.77/35011;
2D   -!-   PI/MW: SPOT 2D-001EDR=4.82/34718;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001EDQ: 1568.72; 1583.84; 2160.96; 2243.2;
2D         2259.17; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001EDR: 1568.71; 1583.85; 2160.98; 2227.15;
2D         2243.2; 2259.16; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
2D   -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001VPE=4.79/38894;
2D   -!-   PI/MW: SPOT 2D-001VQC=4.68/38388;
2D   -!-   PI/MW: SPOT 2D-001VR9=4.79/37314;
2D   -!-   PI/MW: SPOT 2D-001VRG=4.65/36910;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001VPE: 910.5; 931.5; 974.5; 1023.5;
2D         1568.5; 2160; 2227.2; 2243.2; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001VQC: 910.5; 931.5; 974.6; 1023.6;
2D         1360.8; 1568.9; 2145.2; 2161.2; 2222.4; 2243.4; 2945.8; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001VR9: 974.5; 1023.5; 1568.7; 2227.2;
2D         2243.2; 2945.4; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001VRG: 910.4; 931.4; 974.5; 1023.5;
2D         1360.6; 1568.7; 1835.8; 2144.9; 2160.9; 2227.2; 2243.1; 2929.4; 2945.4; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001VR9: (K)MoxSVQPTVSLGGFEITPPVVLR(L),81-101.
2D   -!-   MAPPING: Peptide mass fingerprinting [2]; SPOT 2D-001VR9: TANDEM
2D         MASS SPECTROMETRY [2].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   DOSAC-COBS 2D-PAGE; P06748; P06748.
DR   DOSAC-COBS-2DPAGE; P06748; NPM_HUMAN.
DR   REPRODUCTION-2DPAGE; IPI00549248; IPI00549248.
DR   UniProtKB/Swiss-Prot; P06748; NPM_HUMAN.
//


The following 2-D maps are available for this protein:
Colorectal adenocarcinoma cell line (DL-1)
DLD1_HUMAN
SDS-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_1D_HUMAN
2D-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_2D_HUMAN


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server