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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P06999




SWISS-2DPAGE:  P06999


P06999


General information about the entry
View entry in simple text format
Entry nameK6PF2_ECOLI
Primary accession numberP06999
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=6-phosphofructokinase isozyme 2; Short=Phosphofructokinase-2; EC=2.7.1.11;.
Gene nameName=pfkB
OrderedLocusNames=b1723, JW5280
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
MEDLINE=83270974; PubMed=6308410; [NCBI, Expasy, EBI, Israel, Japan]
Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.
''''''Gene-protein index of Escherichia coli K-12'';'';''
Microbiol. Rev. 47(1):231-284(1983)
[3]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.30; Mw=38081

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT [2] AND VANBOGELEN [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP06999; K6PF2_ECOLI.
ECO2DBASEE036.6; 6TH EDITION.
UniProtKB/Swiss-ProtP06999; K6PF2_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameK6PF2_ECOLI
Primary accession numberP06999
Secondary accession number(s) P78065 P78260
Sequence was last modified on November 1, 1997 (version 2)
Annotations were last modified on October 19, 2011 (version 103)
Name and origin of the protein
DescriptionRecName: Full=6-phosphofructokinase isozyme 2; Short=Phosphofructokinase-2; EC=2.7.1.11;
Gene nameName=pfkB
OrderedLocusNames=b1723, JW5280
Encoded onName=pfkB; OrderedLocusNames=b1723, JW5280
Keywords3D-structure; ATP-binding; Complete proteome; Glycolysis; Kinase; Nucleotide-binding; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLK02500; AAA24321.1; -; Genomic_DNA
EMBLU00096; AAC74793.1; -; Genomic_DNA
EMBLAP009048; BAA15500.2; -; Genomic_DNA
EMBLK00128; AAA24320.1; -; Genomic_DNA
PIRC64931; KIECFB; .
RefSeqNP_416237.3; NC_000913.2; .
PDB3CQD; X-ray; 1.98 A; A/B=1-309
PDB3N1C; X-ray; 2.00 A; A/B/C/D=1-309
PDBsum3CQD; -; .
PDBsum3N1C; -; .
ProteinModelPortalP06999; -; .
SMRP06999; 1-308; .
DIPDIP-10465N; -; .
SWISS-2DPAGEP06999; -; .
2DBase-EcoliP06999; -; .
ECO2DBASEE036.6; 6TH EDITION; .
PRIDEP06999; -; .
EnsemblBacteriaEBESCT00000000713; EBESCP00000000713; EBESCG00000000594; .
EnsemblBacteriaEBESCT00000000714; EBESCP00000000714; EBESCG00000000594; .
EnsemblBacteriaEBESCT00000017556; EBESCP00000016847; EBESCG00000016612; .
GeneID946230; -; .
GenomeReviewsAP009048_GR; JW5280; .
GenomeReviewsU00096_GR; b1723; .
KEGGecj:JW5280; -; .
KEGGeco:b1723; -; .
EchoBASEEB0694; -; .
EcoGeneEG10700; pfkB; .
eggNOGCOG1105; -; .
GeneTreeEBGT00070000031691; -; .
HOGENOMHBG670529; -; .
OMAVYLVKPS; -; .
ProtClustDBPRK10294; -; .
BioCycEcoCyc:6PFK-2-MONOMER; -; .
BioCycMetaCyc:6PFK-2-MONOMER; -; .
GenevestigatorP06999; -; .
GOGO:0003872; F:6-phosphofructokinase activity; IEA:EC; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004616; F:phosphogluconate dehydrogenase (decarboxylating) activity; IMP:EcoliWiki; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterProIPR011611; Carb/pur_kinase; .
InterProIPR002173; Carboh/pur_kinase_PfkB_CS; .
InterProIPR017583; Tagatose/fructose_Pkinase; .
PfamPF00294; PfkB; 1; .
TIGRFAMsTIGR03168; 1-PFK; 1; .
PROSITEPS00583; PFKB_KINASES_1; 1; .
PROSITEPS00584; PFKB_KINASES_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server