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SWISS-2DPAGE

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It will be discontinued on 31-May-2024.

Swiss-2DPAGE data (text records and image files) will continue to be available from https://ftp.expasy.org/databases/swiss-2dpage/.


SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P07195




SWISS-2DPAGE:  P07195


P07195


General information about the entry
View entry in simple text format
Entry nameLDHB_HUMAN
Primary accession numberP07195
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 18)
Name and origin of the protein
DescriptionRecName: Full=L-lactate dehydrogenase B chain; Short=LDH-B; EC=1.1.1.27; AltName: Full=LDH heart subunit; Short=LDH-H; AltName: Full=Renal carcinoma antigen NY-REN-46;.
Gene nameName=LDHB
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=79188222; PubMed=109131; [NCBI, Expasy, EBI, Israel, Japan]
Edwards J., Anderson N.G., Nance S.L., Anderson N.L.
''''''Red cell proteins. I. Two-dimensional mapping of human erythrocyte lysate proteins'';'';''
Blood 53(1):1121-1132(1979)
[2]   MAPPING ON GEL
MEDLINE=96007935; PubMed=7498158; [NCBI, Expasy, EBI, Israel, Japan]
Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.
''''''Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis'';'';''
Electrophoresis 16(1):1152-1159(1995)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

PLATELET_HUMAN {Platelet}
Homo sapiens (Human)
Tissue: Platelet
PLATELET_HUMAN
  map experimental info
  protein estimated location
 
PLATELET_HUMAN

MAP LOCATIONS:
pI=5.66; Mw=34762

MAPPING (identification):
MATCHING WITH THE RBC MASTER GEL [2].



RBC_HUMAN {Red blood cells}
Homo sapiens (Human)
Tissue: Erythrocyte
RBC_HUMAN
  map experimental info
  protein estimated location
 
RBC_HUMAN

MAP LOCATIONS:
pI=5.71; Mw=33868

MAPPING (identification):
IMMUNOBLOTTING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
DOSAC-COBS 2D-PAGEP07195; P07195.
DOSAC-COBS-2DPAGEP07195; LDHB_HUMAN.
HSC-2DPAGEP07195; HUMAN.
OGP-WWWP07195.
REPRODUCTION-2DPAGEIPI00219217; IPI00219217.
UCD-2DPAGEP07195; LDHB_HUMAN.
UniProtKB/Swiss-ProtP07195; LDHB_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameLDHB_HUMAN
Primary accession numberP07195
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 146)
Name and origin of the protein
DescriptionRecName: Full=L-lactate dehydrogenase B chain; Short=LDH-B; EC=1.1.1.27; AltName: Full=LDH heart subunit; Short=LDH-H; AltName: Full=Renal carcinoma antigen NY-REN-46;
Gene nameName=LDHB
Encoded onName=LDHB
Keywords3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Glycolysis; NAD; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX13794; CAA32033.1; -; Genomic_DNA
EMBLX13795; CAA32033.1; JOINED; Genomic_DNA
EMBLX13796; CAA32033.1; JOINED; Genomic_DNA
EMBLX13797; CAA32033.1; JOINED; Genomic_DNA
EMBLX13798; CAA32033.1; JOINED; Genomic_DNA
EMBLX13799; CAA32033.1; JOINED; Genomic_DNA
EMBLX13800; CAA32033.1; JOINED; Genomic_DNA
EMBLY00711; CAA68701.1; -; mRNA
EMBLBC002362; AAH02362.1; -; mRNA
EMBLBC015122; AAH15122.1; -; mRNA
EMBLBC071860; AAH71860.1; -; mRNA
IPIIPI00219217; -; .
PIRS02795; DEHULH; .
RefSeqNP_001167568.1; NM_001174097.1; .
RefSeqNP_002291.1; NM_002300.6; .
UniGeneHs.446149; -; .
PDB1I0Z; X-ray; 2.10 A; A/B=2-334
PDB1T2F; X-ray; 3.00 A; A/B/C/D=2-332
PDBsum1I0Z; -; .
PDBsum1T2F; -; .
ProteinModelPortalP07195; -; .
SMRP07195; 2-333; .
IntActP07195; 6; .
MINTMINT-1144561; -; .
STRINGP07195; -; .
PhosphoSiteP07195; -; .
SWISS-2DPAGEP07195; -; .
Cornea-2DPAGEP07195; -; .
DOSAC-COBS-2DPAGEP07195; -; .
OGPP07195; -; .
REPRODUCTION-2DPAGEIPI00219217; -; .
UCD-2DPAGEP07195; -; .
PRIDEP07195; -; .
EnsemblENST00000350669; ENSP00000229319; ENSG00000111716; .
EnsemblENST00000396076; ENSP00000379386; ENSG00000111716; .
GeneID3945; -; .
KEGGhsa:3945; -; .
UCSCuc001rfc.2; human; .
CTD3945; -; .
GeneCardsGC12M021561; -; .
H-InvDBHIX0010481; -; .
HGNCHGNC:6541; LDHB; .
HPACAB004641; -; .
MIM150100; gene; .
MIM614128; phenotype; .
neXtProtNX_P07195; -; .
Orphanet2364; Lactate dehydrogenase deficiency; .
PharmGKBPA30325; -; .
eggNOGprNOG09366; -; .
HOGENOMHBG566126; -; .
HOVERGENHBG000462; -; .
InParanoidP07195; -; .
OMAYSPDCTI; -; .
OrthoDBEOG4RR6HR; -; .
PhylomeDBP07195; -; .
ReactomeREACT_1046; Pyruvate metabolism and Citric Acid (TCA) cycle; .
BindingDBP07195; -; .
DrugBankDB00157; NADH; .
NextBio15479; -; .
ArrayExpressP07195; -; .
BgeeP07195; -; .
CleanExHS_LDHB; -; .
GenevestigatorP07195; -; .
GermOnlineENSG00000111716; Homo sapiens; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0004459; F:L-lactate dehydrogenase activity; TAS:ProtInc; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
GOGO:0006090; P:pyruvate metabolic process; TAS:Reactome; .
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR011304; L-lactate_DH; .
InterProIPR018177; L-lactate_DH_AS; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
PRINTSPR00086; LLDHDRGNASE; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMsTIGR01771; L-LDH-NAD; 1; .
PROSITEPS00064; L_LDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server