World-2DPAGE Home
Home  |  Contact

Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P08113




SWISS-2DPAGE:  P08113


P08113


General information about the entry
View entry in simple text format
Entry nameENPL_MOUSE
Primary accession numberP08113
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Endoplasmin; AltName: Full=94 kDa glucose-regulated protein; Short=GRP-94; AltName: Full=Endoplasmic reticulum resident protein 99; Short=ERp99; AltName: Full=Heat shock protein 90 kDa beta member 1; AltName: Full=Polymorphic tumor rejection antigen 1; AltName: Full=Tumor rejection antigen gp96; Flags: Precursor;.
Gene nameName=Hsp90b1
Synonyms=Grp94, Tra-1, Tra1
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96154592; PubMed=8586073; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers J., Steiner S., Witzmann F., Anderson N.G.
''''''An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies'';'';''
Electrophoresis 16(1):1977-1981(1995)
[2]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=4.92; Mw=88521

MAPPING (identification):
GEL MATCHING WITH WAT_MOUSE [2].



ISLETS_MOUSE {Pancreatic islet cells}
Mus musculus (Mouse)
Tissue: Pancreatic islet
ISLETS_MOUSE
  map experimental info
  protein estimated location
 
ISLETS_MOUSE

MAP LOCATIONS:
pI=4.93; Mw=104271  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=4.87; Mw=90604
pI=4.88; Mw=89679
pI=4.89; Mw=90000

MAPPING (identification):
MATCHING [2] WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=4.86; Mw=96873

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=4.91; Mw=90000

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
REPRODUCTION-2DPAGEP08113; P08113.
UniProtKB/Swiss-ProtP08113; ENPL_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameENPL_MOUSE
Primary accession numberP08113
Secondary accession number(s) P11427
Sequence was last modified on April 1, 1990 (version 2)
Annotations were last modified on September 21, 2011 (version 125)
Name and origin of the protein
DescriptionRecName: Full=Endoplasmin; AltName: Full=94 kDa glucose-regulated protein; Short=GRP-94; AltName: Full=Endoplasmic reticulum resident protein 99; Short=ERp99; AltName: Full=Heat shock protein 90 kDa beta member 1; AltName: Full=Polymorphic tumor rejection antigen 1; AltName: Full=Tumor rejection antigen gp96; Flags: Precursor;
Gene nameName=Hsp90b1
Synonyms=Grp94, Tra-1, Tra1
Encoded onName=Hsp90b1; Synonyms=Grp94, Tra-1, Tra1
KeywordsATP-binding; Calcium; Chaperone; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Nucleotide-binding; Phosphoprotein; Reference proteome; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ03297; AAA37573.1; -; mRNA
EMBLM29652; AAA37743.1; -; mRNA
EMBLM16370; AAA40023.1; -; mRNA
IPIIPI00129526; -; .
PIRA29317; A29317; .
RefSeqNP_035761.1; NM_011631.1; .
UniGeneMm.87773; -; .
ProteinModelPortalP08113; -; .
SMRP08113; 72-755; .
DIPDIP-32352N; -; .
IntActP08113; 2; .
STRINGP08113; -; .
PhosphoSiteP08113; -; .
SWISS-2DPAGEP08113; -; .
REPRODUCTION-2DPAGEP08113; -; .
PRIDEP08113; -; .
EnsemblENSMUST00000020238; ENSMUSP00000020238; ENSMUSG00000020048; .
GeneID22027; -; .
KEGGmmu:22027; -; .
CTD7184; -; .
MGIMGI:98817; Hsp90b1; .
eggNOGroNOG05593; -; .
HOGENOMHBG631012; -; .
HOVERGENHBG007374; -; .
InParanoidP08113; -; .
OMAKEKNMLH; -; .
OrthoDBEOG4Z62N4; -; .
PhylomeDBP08113; -; .
NextBio301772; -; .
PMAP-CutDBP08113; -; .
ArrayExpressP08113; -; .
BgeeP08113; -; .
CleanExMM_HSP90B1; -; .
GenevestigatorP08113; -; .
GermOnlineENSMUSG00000020048; Mus musculus; .
GOGO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell; .
GOGO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GOGO:0044459; C:plasma membrane part; IDA:MGI; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0051082; F:unfolded protein binding; IEA:InterPro; .
GOGO:0030433; P:ER-associated protein catabolic process; ISS:UniProtKB; .
GOGO:0006457; P:protein folding; IEA:InterPro; .
InterProIPR003594; ATPase-like_ATP-bd; .
InterProIPR015566; Endoplasmin; .
InterProIPR019805; Heat_shock_protein_90_CS; .
InterProIPR001404; Hsp90; .
InterProIPR020575; Hsp90_N; .
InterProIPR020568; Ribosomal_S5_D2-typ_fold; .
Gene3DG3DSA:3.30.565.10; ATP_bd_ATPase; 2; .
PANTHERPTHR11528:SF21; Endoplasmin; 1; .
PANTHERPTHR11528; Hsp90; 1; .
PfamPF02518; HATPase_c; 1; .
PfamPF00183; HSP90; 1; .
PIRSFPIRSF002583; Hsp90; 1; .
PRINTSPR00775; HEATSHOCK90; .
SMARTSM00387; HATPase_c; 1; .
SUPFAMSSF55874; ATP_bd_ATPase; 1; .
SUPFAMSSF54211; Ribosomal_S5_D2-typ_fold; 1; .
PROSITEPS00014; ER_TARGET; 1; .
PROSITEPS00298; HSP90; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server