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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P08200 |
SWISS-2DPAGE: P08200
P08200:
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ID IDH_ECOLI; STANDARD; 2DG.
AC P08200;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 14.
DE RecName: Full=Isocitrate dehydrogenase [NADP]; Short=IDH; EC=1.1.1.42;
DE AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName:
DE Full=Oxalosuccinate decarboxylase;.
GN Name=icd; Synonyms=icdA, icdE; OrderedLocusNames=b1136, JW1122;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=81069671; PubMed=7002901; [NCBI, Expasy, EBI, Israel, Japan]
RA Phillips T.A., Bloch P.L., Neidhardt F.C.;
RT ''''''Protein identifications on O'Farrell two-dimensional gels:
RT locations of 55 additional Escherichia coli proteins'';'';'';
RL J. Bacteriol. 144(1):1024-1033(1980).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [5]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: MONOMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KVS=5.02/46142;
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D [2] AND PHILLIPS [3].
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HM1=4.70/22389; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001HM1: 1008.518; 1030.514; 1206.644;
2D 1470.789; 1630.906; 1646.858; 1788.869; 2086.112; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0014YW=5.15/43958;
2D -!- PEPTIDE MASSES: SPOT 2D-0014YW: 1030.419; 1124.567; 1206.55;
2D 1373.612; 1662.74; 2085.952; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001KVT=5.00/74518;
2D -!- PEPTIDE MASSES: SPOT 2D-001KVT: 1008.45; 1030.433; 1124.616;
2D 1206.587; 1357.692; 1470.752; 1484.719; 1553.721; 1630.906; 1646.851;
2D 1989.021; 2086.181; 2202.183; 2304.309; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WKP=5.11/44335;
2D -!- PI/MW: SPOT 2D-001WKS=5.15/44053;
2D -!- PEPTIDE MASSES: SPOT 2D-001WKP: 906.44; 1030.49; 1124.65; 1139.61;
2D 1206.65; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WKS: 1124.73; 1139.57; 1206.66; 1357.68;
2D 1373.63; 1646.87; 1662.91; 1674.96; 2045.85; 2085.91; TRYPSIN.
2D -!- EXPRESSION: decrease after benzoic acid treatment [5].
2D -!- MAPPING: Peptide mass fingerprinting [5].
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DR 2DBase-Ecoli; P08200; IDH_ECOLI.
DR ECO2DBASE; C043.8; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P08200; IDH_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4-5)
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Escherichia coli(4.5-5.5)
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Escherichia coli(5-6)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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