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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P08312




SWISS-2DPAGE: P08312



P08312:

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ID   SYFA_ECOLI; STANDARD; 2DG.
AC   P08312;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   01-OCT-2001, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Phenylalanyl-tRNA synthetase alpha chain; EC=6.1.1.20;
DE   AltName: Full=Phenylalanine--tRNA ligase alpha chain; Short=PheRS;.
GN   Name=pheS; OrderedLocusNames=b1714, JW5277;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5.5-6.7, ECOLI5-6.
IM   ECOLI, ECOLI5.5-6.7, ECOLI5-6.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
RA   Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT   ''''''Protein identifications of O'Farrell two-dimensional gels:
RT   locations of 81 Escherichia coli proteins'';'';'';
RL   J. Bacteriol. 141(1):1409-1420(1980).
RN   [4]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=79216190; PubMed=156716; [NCBI, Expasy, EBI, Israel, Japan]
RA   Herendeen S.L., Vanbogelen R.A., Neidhardt F.C.;
RT   ''''''Levels of major proteins of Escherichia coli during growth at
RT   different temperatures'';'';'';
RL   J. Bacteriol. 139(1):185-194(1979).
RN   [5]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=78212912; PubMed=352533; [NCBI, Expasy, EBI, Israel, Japan]
RA   Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.;
RT   ''''''Patterns of protein synthesis in E. coli: a catalog of the amount
RT   of 140 individual proteins at different growth rates'';'';'';
RL   Cell 14(1):179-190(1978).
RN   [6]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
CC   -!- SUBUNIT: TETRAMER OF TWO ALPHA AND TWO BETA CHAINS 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000L2X=5.85/38765;
2D   -!-   EXPRESSION: CHANGED VERY LITTLE THROUGHOUT THE NORMAL ARRHENIUS
2D         TEMPERATURE RANGE (23 TO 37°C) [4]; INCREASED IN LEVEL WITH INCREASING
2D         GROWTH RATE [5].
2D   -!-   MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D         [2] AND BLOCH [3].
2D   -!- MASTER: ECOLI5.5-6.7;
2D   -!-   PI/MW: SPOT 2D-001UBR=5.88/40109;
2D   -!-   MAPPING: GEL MATCHING WITH ECOLI5-6 [6].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001L8E=5.81/45820;
2D   -!-   PI/MW: SPOT 2D-001LAE=5.74/40430;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L8E: 823.451; 958.517; 989.555;
2D         1027.506; 1156.618; 1272.692; 1310.654; 1341.728; 1600.819; 1890.936;
2D         2320.031; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LAE: 823.408; 958.481; 989.518; 1027.49;
2D         1156.593; 1192.651; 1310.64; 1341.73; 1600.814; 1890.979; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [6].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; G036.0; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P08312; SYFA_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(5.5-6.7)
ECOLI5.5-6.7
Escherichia coli(5-6)
ECOLI5-6


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server