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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P09551 |
SWISS-2DPAGE: P09551
P09551:
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ID ARGT_ECOLI; STANDARD; 2DG.
AC P09551;
DT 01-OCT-1998, integrated into SWISS-2DPAGE (release 8).
DT 15-MAY-2003, 2D annotation version 2.
DT 19-MAY-2011, general annotation version 14.
DE RecName: Full=Lysine-arginine-ornithine-binding periplasmic protein;
DE Short=LAO-binding protein; Flags: Precursor;.
GN Name=argT; OrderedLocusNames=b2310, JW2307;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [2]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0015AC=5.17/26913;
2D -!- PEPTIDE MASSES: SPOT 2D-0015AC: 1140.99; 1776.18; 2196.32; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1], and microsequencing
2D (ALPE) [1].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WT8=5.15/26055;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WT8: (K)IDAIISSLSITDK(R),86-98;
2D (K)IDAIISSLSITDKR(Q),86-99; (R)QQEIAFSDK(L),100-108;
2D (K)GSPIQPTLDSLK(G),121-132; (K)GVDVVAYANQDLVYSDLAAGR(L),156-176;
2D (R)LDAALQDEVAASEGFLK(Q),177-193; (K)YFGDGTGVGLR(K),212-222; (R)KDDAELTAAFNK(A),223-234.
2D -!- EXPRESSION: decrease after benzoic acid treatment [2].
2D -!- MAPPING: Tandem mass spectrometry [2].
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DR 2DBase-Ecoli; P09551; ARGT_ECOLI.
DR UniProtKB/Swiss-Prot; P09551; ARGT_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli(4.5-5.5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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