World-2DPAGE Home
Home  |  Contact

Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P09832




SWISS-2DPAGE: P09832



P09832:

Nice View - a user-friendly view of this entry
ID   GLTD_ECOLI; STANDARD; 2DG.
AC   P09832;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   01-OCT-2001, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Glutamate synthase [NADPH] small chain; EC=1.4.1.13;
DE   AltName: Full=Glutamate synthase subunit beta; Short=GLTS beta chain;
DE   AltName: Full=NADPH-GOGAT;.
GN   Name=gltD; Synonyms=aspB; OrderedLocusNames=b3213, JW3180;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6.
IM   ECOLI, ECOLI5-6.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=83270974; PubMed=6308410; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.;
RT   ''''''Gene-protein index of Escherichia coli K-12'';'';'';
RL   Microbiol. Rev. 47(1):231-284(1983).
RN   [3]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [4]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [5]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=92138602; PubMed=1346534; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Ernsting B.R., Atkinson M.R., Ninfa A.J., Matthews R.G.;
RT   ''''''Characterization of the regulon controlled by the
RT   leucine-responsive regulatory protein in Escherichia coli'';'';'';
RL   J. Bacteriol. 174(1):1109-1118(1992).
RN   [6]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
CC   -!- SUBUNIT: AGGREGATE OF 4 CATALYTICAL ACTIVE HETERODIMERS 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KQL=5.40/52658;
2D   -!-   PI/MW: SPOT 2D-000KQS=5.47/52555;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000KQL: B=11.2, Z=12, S=5.2, H=1,
2D         G=13.2, T=4.4, A=9.7, P=4.9, Y=1.3, R=7.4, V=7.7, M=2.2, I=4.9, L=6.9,
2D         F=4.9, K=3.1;
2D   -!-   EXPRESSION: AN APPROXIMATELY TWO-FOLD-LOWER GLUTAMATE SYNTHASE
2D         ACTIVITY WAS DETECTED WHEN LRP+ STRAINS WERE GROWN IN THE PRESENCE OF
2D         EXOGENOUS LEUCINE [5].
2D   -!-   MAPPING: SPOT 2D-000KQL: AMINO ACID COMPOSITION AND SEQUENCE TAG
2D         (SQNV) [4]; SPOT 2D-000KQS: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS
2D         BY NEIDHARDT [2] AND VANBOGELEN [3].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001KYZ=5.48/53050;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001KYZ: 906.441; 947.543; 977.467;
2D         1038.516; 1054.509; 1127.56; 1206.539; 1271.66; 1287.698; 1510.78;
2D         1564.75; 1610.753; 1626.757; 1642.754; 1767.996; 1788.879; 1825.007;
2D         1953.141; 2014.014; 2272.117; 2455.135; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [6].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; F050.4; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P09832; GLTD_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(5-6)
ECOLI5-6


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server