Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

P0A6D7:

ID   AROK_ECOLI; STANDARD; 2DG.
AC   P0A6D7; P24167;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Shikimate kinase 1; Short=SK 1; EC=2.7.1.71; AltName:
DE   Full=Shikimate kinase I; Short=SKI;.
GN   Name=aroK; OrderedLocusNames=b3390, JW5947;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000LNO=5.29/17936;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (AEKR) [1].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LOE=5.18/21250;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LOE: 945.483; 1143.611; 1314.784;
2D         1350.775; 1442.905; 1483.769; 1532.855; 2485.251; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WWI=5.30/19078;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WWI: 1143.62; 1186.69; 1203.67; 1219.66;
2D         1314.8; 1350.77; 1442.91; 1483.73; 1532.82; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [3].
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DR   UniProtKB/Swiss-Prot; P0A6D7; AROK_ECOLI.
//

The following 2-D maps are available for this protein:

	Escherichia coli	Escherichia coli(5-6)	Escherichia coli DIGE (4.5-6.5)

SWISS-2DPAGE (search AC)