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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A6E4




SWISS-2DPAGE:  P0A6E4


P0A6E4


General information about the entry
View entry in simple text format
Entry nameASSY_ECOLI
Primary accession numberP0A6E4
Secondary accession number(s) P22767
integrated into SWISS-2DPAGE on October 1, 1998 (release 8)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Argininosuccinate synthase; EC=6.3.4.5; AltName: Full=Citrulline--aspartate ligase;.
Gene nameName=argG
OrderedLocusNames=b3172, JW3140
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[2]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.28; Mw=46220  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.08; Mw=51667  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.27; Mw=46667  [identification data]

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0A6E4; ASSY_ECOLI.
UniProtKB/Swiss-ProtP0A6E4; ASSY_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameASSY_ECOLI
Primary accession numberP0A6E4
Secondary accession number(s) P22767 Q2M939
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 68)
Name and origin of the protein
DescriptionRecName: Full=Argininosuccinate synthase; EC=6.3.4.5; AltName: Full=Citrulline--aspartate ligase;
Gene nameName=argG
OrderedLocusNames=b3172, JW3140
Encoded onName=argG; OrderedLocusNames=b3172, JW3140
Keywords3D-structure; Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM35236; AAA23482.1; -; Genomic_DNA
EMBLU18997; AAA57974.1; -; Genomic_DNA
EMBLU00096; AAC76205.1; -; Genomic_DNA
EMBLAP009048; BAE77217.1; -; Genomic_DNA
PIRG65107; AJECRS; .
RefSeqNP_417640.1; NC_000913.2; .
PDB1K92; X-ray; 1.60 A; A=2-447
PDB1K97; X-ray; 2.00 A; A=2-447
PDB1KP2; X-ray; 2.00 A; A=2-447
PDB1KP3; X-ray; 2.00 A; A=2-447
PDBsum1K92; -; .
PDBsum1K97; -; .
PDBsum1KP2; -; .
PDBsum1KP3; -; .
ProteinModelPortalP0A6E4; -; .
SMRP0A6E4; 2-445; .
DIPDIP-35842N; -; .
IntActP0A6E4; 13; .
SWISS-2DPAGEP0A6E4; -; .
2DBase-EcoliP0A6E4; -; .
EnsemblBacteriaEBESCT00000003638; EBESCP00000003638; EBESCG00000002971; .
EnsemblBacteriaEBESCT00000017420; EBESCP00000016711; EBESCG00000016476; .
GeneID947590; -; .
GenomeReviewsAP009048_GR; JW3140; .
GenomeReviewsU00096_GR; b3172; .
KEGGecj:JW3140; -; .
KEGGeco:b3172; -; .
EchoBASEEB0066; -; .
EcoGeneEG10068; argG; .
eggNOGCOG0137; -; .
GeneTreeEBGT00050000011822; -; .
HOGENOMHBG289560; -; .
OMAGGRKEMS; -; .
ProtClustDBPRK05370; -; .
BioCycEcoCyc:ARGSUCCINSYN-MONOMER; -; .
BioCycMetaCyc:ARGSUCCINSYN-MONOMER; -; .
DrugBankDB00536; Guanidine; .
GenevestigatorP0A6E4; -; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0004055; F:argininosuccinate synthase activity; EXP:EcoCyc; .
GOGO:0005524; F:ATP binding; EXP:EcoCyc; .
GOGO:0042803; F:protein homodimerization activity; IEA:InterPro; .
GOGO:0006526; P:arginine biosynthetic process; IEA:UniProtKB-KW; .
HAMAPMF_00581; Arg_succ_synth_type2; 1; -
InterProIPR023437; Arg_succ_synth_type2_subfam; .
InterProIPR001518; Arginosuc_synth; .
InterProIPR018223; Arginosuc_synth_CS; .
InterProIPR024074; AS_cat/multimer_dom_body; .
InterProIPR024073; AS_multimer_C_tail; .
InterProIPR014729; Rossmann-like_a/b/a_fold; .
Gene3DG3DSA:1.10.287.400; G3DSA:1.10.287.400; 1; .
Gene3DG3DSA:3.90.1260.10; G3DSA:3.90.1260.10; 1; .
Gene3DG3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1; .
PANTHERPTHR11587; Arginosuc_synth; 1; .
PfamPF00764; Arginosuc_synth; 1; .
TIGRFAMsTIGR00032; ArgG; 1; .
PROSITEPS00564; ARGININOSUCCIN_SYN_1; 1; .
PROSITEPS00565; ARGININOSUCCIN_SYN_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server