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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A6F1




SWISS-2DPAGE:  P0A6F1


P0A6F1


General information about the entry
View entry in simple text format
Entry nameCARA_ECOLI
Primary accession numberP0A6F1
Secondary accession number(s) P00907
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Carbamoyl-phosphate synthase small chain; EC=6.3.5.5; AltName: Full=Carbamoyl-phosphate synthetase glutamine chain;.
Gene nameName=carA
Synonyms=pyrA
OrderedLocusNames=b0032, JW0030
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[4]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: HETERODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.91; Mw=44176
pI=5.94; Mw=43914  [identification data]

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2];
SPOT 2D-000KXV: AMINO ACID COMPOSITION AND SEQUENCE TAG (MIK) [3].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.88; Mw=42128  [identification data]
pI=5.82; Mw=42128  [identification data]
pI=5.79; Mw=41417  [identification data]

MAPPING (identification):
SPOT 2D-001WM0: PEPTIDE MASS FINGERPRINTING [4]; Tandem mass spectrometry [4].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEG041.4; 6TH EDITION.
UniProtKB/Swiss-ProtP0A6F1; CARA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCARA_ECOLI
Primary accession numberP0A6F1
Secondary accession number(s) P00907
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 72)
Name and origin of the protein
DescriptionRecName: Full=Carbamoyl-phosphate synthase small chain; EC=6.3.5.5; AltName: Full=Carbamoyl-phosphate synthetase glutamine chain;
Gene nameName=carA
Synonyms=pyrA
OrderedLocusNames=b0032, JW0030
Encoded onName=carA; Synonyms=pyrA; OrderedLocusNames=b0032, JW0030
Keywords3D-structure; Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; Complete proteome; Direct protein sequencing; Glutamine amidotransferase; Ligase; Nucleotide-binding; Pyrimidine biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ01597; AAA23538.1; -; Genomic_DNA
EMBLU00096; AAC73143.1; -; Genomic_DNA
EMBLAP009048; BAB96601.1; -; Genomic_DNA
EMBLX70017; CAA49615.1; -; Genomic_DNA
PIRA01128; SYECCS; .
PIRB85484; B85484; .
RefSeqNP_414573.1; NC_000913.2; .
PDB1A9X; X-ray; 1.80 A; B/D/F/H=2-380
PDB1BXR; X-ray; 2.10 A; B/D/F/H=1-382
PDB1C30; X-ray; 2.00 A; B/D/F/H=1-382
PDB1C3O; X-ray; 2.10 A; B/D/F/H=1-382
PDB1CE8; X-ray; 2.10 A; B/D/F/H=1-382
PDB1CS0; X-ray; 2.00 A; B/D/F/H=1-382
PDB1JDB; X-ray; 2.10 A; C/F/I/L=1-382
PDB1KEE; X-ray; 2.10 A; B/D/F/H=1-382
PDB1M6V; X-ray; 2.10 A; B/D/F/H=1-382
PDB1T36; X-ray; 2.10 A; B/D/F/H=1-382
PDBsum1A9X; -; .
PDBsum1BXR; -; .
PDBsum1C30; -; .
PDBsum1C3O; -; .
PDBsum1CE8; -; .
PDBsum1CS0; -; .
PDBsum1JDB; -; .
PDBsum1KEE; -; .
PDBsum1M6V; -; .
PDBsum1T36; -; .
ProteinModelPortalP0A6F1; -; .
SMRP0A6F1; 2-380; .
DIPDIP-35412N; -; .
IntActP0A6F1; 15; .
SWISS-2DPAGEP0A6F1; -; .
ECO2DBASEG041.4; 6TH EDITION; .
EnsemblBacteriaEBESCT00000002116; EBESCP00000002116; EBESCG00000001734; .
EnsemblBacteriaEBESCT00000002117; EBESCP00000002117; EBESCG00000001734; .
EnsemblBacteriaEBESCT00000016355; EBESCP00000015646; EBESCG00000015415; .
GeneID949025; -; .
GenomeReviewsAP009048_GR; JW0030; .
GenomeReviewsU00096_GR; b0032; .
KEGGecj:JW0030; -; .
KEGGeco:b0032; -; .
EchoBASEEB0132; -; .
EcoGeneEG10134; carA; .
eggNOGCOG0505; -; .
GeneTreeEBGT00050000010377; -; .
HOGENOMHBG286341; -; .
OMAFTYPELG; -; .
ProtClustDBPRK12564; -; .
BioCycEcoCyc:CARBPSYN-SMALL; -; .
BioCycMetaCyc:CARBPSYN-SMALL; -; .
GenevestigatorP0A6F1; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004088; F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; IEA:EC; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0006207; P:'de novo' pyrimidine base biosynthetic process; IMP:EcoliWiki; .
GOGO:0006526; P:arginine biosynthetic process; IMP:EcoliWiki; .
GOGO:0006541; P:glutamine metabolic process; IMP:EcoliWiki; .
GOGO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:UniProtKB-KW; .
HAMAPMF_01209; CPSase_S_chain; 1; -
InterProIPR006220; Anth_synthII; .
InterProIPR001317; CarbamoylP_synth_GATase_dom; .
InterProIPR006274; CarbamoylP_synth_ssu; .
InterProIPR002474; CarbamoylP_synth_ssu_N; .
InterProIPR011702; GATASE; .
InterProIPR017926; GATASE_1; .
Gene3DG3DSA:3.50.30.20; G3DSA:3.50.30.20; 1; .
PfamPF00988; CPSase_sm_chain; 1; .
PfamPF00117; GATase; 1; .
PRINTSPR00097; ANTSNTHASEII; .
PRINTSPR00099; CPSGATASE; .
PRINTSPR00096; GATASE; .
SUPFAMSSF52021; CP_synthsmall; 1; .
TIGRFAMsTIGR01368; CPSaseIIsmall; 1; .
PROSITEPS51273; GATASE_TYPE_1; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server