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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A6F1




SWISS-2DPAGE: P0A6F1



P0A6F1:

Nice View - a user-friendly view of this entry
ID   CARA_ECOLI; STANDARD; 2DG.
AC   P0A6F1; P00907;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Carbamoyl-phosphate synthase small chain; EC=6.3.5.5;
DE   AltName: Full=Carbamoyl-phosphate synthetase glutamine chain;.
GN   Name=carA; Synonyms=pyrA; OrderedLocusNames=b0032, JW0030;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: HETERODIMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KXL=5.91/44176;
2D   -!-   PI/MW: SPOT 2D-000KXV=5.94/43914;
2D   -!-   MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D         [2]; SPOT 2D-000KXV: AMINO ACID COMPOSITION AND SEQUENCE TAG (MIK) [3].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WM0=5.88/42128;
2D   -!-   PI/MW: SPOT 2D-001WM3=5.82/42128;
2D   -!-   PI/MW: SPOT 2D-001WMA=5.79/41417;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WM0: 1145.51; 1186.47; 1325.55; 1337.59;
2D         1343.59; 1493.66; 1642.72; 1657.65; 1964.75; 4064.65; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WM0: (K)SALLVLEDGTQFHGR(A),4-18;
2D         (R)NTEDLSSYLK(R),94-103; (K)EVTTAEAYSWTQGSWTLTGGLPEAK(K),161-185;
2D         (K)KEDELPFHVVAYDFGAK(R),186-202; (R)LTIVPAQTSAEDVLK(M),216-230;
2D         (K)FLETDIPVFGICLGHQLLALASGAK(T),258-282; (K)SLFDGTLQGIHR(T),331-342.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WM3: (R)DLPLIASNFR(N),84-93; (R)NTEDLSSYLKR(H),94-104.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WMA: (R)DLPLIASNFR(N),84-93;
2D         (R)LTIVPAQTSAEDVLK(M),216-230; (K)FLETDIPVFGICLGHQLLALASGAK(T),258-282; (K)SLFDGTLQGIHR(T),331-342.
2D   -!-   MAPPING: SPOT 2D-001WM0: PEPTIDE MASS FINGERPRINTING [4]; Tandem
2D         mass spectrometry [4].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; G041.4; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A6F1; CARA_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server