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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A6F5




SWISS-2DPAGE: P0A6F5



P0A6F5:

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ID   CH60_ECOLI; STANDARD; 2DG.
AC   P0A6F5; P06139;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 14.
DE   RecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName:
DE   Full=Protein Cpn60;.
GN   Name=groL; Synonyms=groEL, mopA; OrderedLocusNames=b4143, JW4103;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=94042879; PubMed=8226654; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Gage D.J., Neidhardt F.C.;
RT   ''''''Adaptation of Escherichia coli to the uncoupler of oxidative
RT   phosphorylation 2,4-dinitrophenol'';'';'';
RL   J. Bacteriol. 175(1):7105-7108(1993).
RN   [4]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=87083376; PubMed=3539918; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Vanbogelen R.A., Kelley P.M., Neidhardt F.C.;
RT   ''''''Differential induction of heat shock, SOS, and oxidation stress
RT   regulons and accumulation of nucleotides in Escherichia coli'';'';'';
RL   J. Bacteriol. 169(1):26-32(1987).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [6]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: OLIGOMER OF 14 SUBUNITS COMPOSED OF TWO STACKED RINGS OF 7
CC         SUBUNITS
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KMU=4.85/56743;
2D   -!-   EXPRESSION: INCREASED FOLLOWING EXPOSURE TO DNP [3]; WAS INDUCED BY
2D         TEMPERATURE SHIFTS, ETHANOL, NALIDIXIC ACID AND PUROMYCIN [4].
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND
2D         IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D   -!- MASTER: ECOLI4-5;
2D   -!-   PI/MW: SPOT 2D-001HFT=4.97/61125;
2D   -!-   PI/MW: SPOT 2D-001HGK=4.99/56877;
2D   -!-   PI/MW: SPOT 2D-001HGR=4.93/55185;
2D   -!-   PI/MW: SPOT 2D-001HGT=4.95/55185;
2D   -!-   PI/MW: SPOT 2D-001HGU=4.95/55185;
2D   -!-   PI/MW: SPOT 2D-001HH2=4.84/52802;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HFT: 875.438; 985.527; 1011.535;
2D         1030.555; 1045.554; 1050.516; 1199.665; 1217.623; 1233.63; 1238.67;
2D         1373.743; 1389.724; 1454.669; 1567.917; 1589.896; 1711.811; 1760.892;
2D         1845.951; 1957.022; 2010.967; 2026.985; 2402.256; 2851.322; 2867.308;
2D         3239.755; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HGK: 875.403; 1011.518; 1045.563;
2D         1238.657; 1373.698; 1454.662; 1567.901; 1711.785; 1769.916; 1798.985;
2D         1845.934; 2010.945; 2026.953; 2399.304; 2402.255; 2851.301; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HGR: 1045.564; 1567.964; 1711.869;
2D         1769.995; 1799.061; 1846.024; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HGT: 1567.875; 1711.773; 1760.854;
2D         1769.935; 1845.925; 2399.269; 2402.224; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HGU: 875.466; 921.509; 985.573; 1011.54;
2D         1030.584; 1045.564; 1199.675; 1201.626; 1217.62; 1238.684; 1373.726;
2D         1389.709; 1454.66; 1567.899; 1589.884; 1711.782; 1760.888; 1845.925;
2D         1956.987; 2010.943; 2026.94; 2402.219; 2851.258; 3239.653; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HH2: 1045.564; 1454.728; 1567.962;
2D         1711.934; 1761.053; 1846.106; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [5]; SPOTS 1HGK, 1HGU: GEL
2D         MATCHING WITH MASTER ECOLI4.5-5.5 [5].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-0014UQ=4.99/55445;
2D   -!-   PI/MW: SPOT 2D-0014V3=4.96/54287;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0014UQ: 921.46; 985.49; 1011.48; 1201.53;
2D         1215.61; 1249.53; 1454.55; 1567.77; 1589.74; 1782.69; 1845.77; 2042.79;
2D         2402.01; 2805.51; 2867.05; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-0014V3: 1011.54; 1201.6; 1454.7; 1567.9;
2D         1846; 2402.3; 2867.4; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [5]; SPOT 2D-0014V3:
2D         MICROSEQUENCING (XLRG) [5].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WFT=4.93/61278;
2D   -!-   PI/MW: SPOT 2D-001WFW=4.96/59987;
2D   -!-   PI/MW: SPOT 2D-001WG2=4.98/60758;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WFT: 921.54; 1201.59; 1454.68; 1567.89;
2D         1711.78; 1760.96; 1845.93; 1957.01; 2042.92; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WFW: 875.39; 1011.46; 1201.53; 1238.52;
2D         1443.6; 1454.51; 1567.73; 1589.69; 1711.6; 1760.69; 1769.71; 1845.72;
2D         1956.77; 2398.94; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WG2: 921.49; 1030.57; 1201.62; 1215.69;
2D         1454.67; 1567.9; 1760.93; 1845.94; 2042.94; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WFW: (K)SFGAPTITK(D),42-50;
2D         (R)GIDKAVTAAVEELK(A),118-131; (K)AVTAAVEELK(A),122-131;
2D         (R)VVINKDTTTIIDGVGEEAAIQGR(V),322-344; (R)AAVEEGVVAGGGVALIR(V),404-420; (K)LADLRGQNEDQNVGIK(V),425-440.
2D   -!-   MAPPING: Peptide mass fingerprinting [6]; SPOT 2D-001WFW: TANDEM
2D         MASS SPECTROMETRY [6].
CC   ---------------------------------------------------------------------------
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CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
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DR   2DBase-Ecoli; P0A6F5; CH60_ECOLI.
DR   ECO2DBASE; B056.5; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A6F5; CH60_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4-5)
ECOLI4-5
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
  


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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server