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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A6H5




SWISS-2DPAGE:  P0A6H5


P0A6H5


General information about the entry
View entry in simple text format
Entry nameHSLU_ECOLI
Primary accession numberP0A6H5
Secondary accession number(s) P32168
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=ATP-dependent protease ATPase subunit HslU; AltName: Full=Heat shock protein HslU; AltName: Full=Unfoldase HslU;.
Gene nameName=hslU
Synonyms=htpI
OrderedLocusNames=b3931, JW3902
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   PROTEIN EXPRESSION
MEDLINE=94042879; PubMed=8226654; [NCBI, ExPASy, EBI, Israel, Japan]
Gage D.J., Neidhardt F.C.
''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';''
J. Bacteriol. 175(1):7105-7108(1993)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.29; Mw=48676

EXPRESSION:
INCREASED FOLLOWING EXPOSURE TO DNP [3].

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASED048.5; 6TH EDITION.
UniProtKB/Swiss-ProtP0A6H5; HSLU_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameHSLU_ECOLI
Primary accession numberP0A6H5
Secondary accession number(s) P32168 Q2M8M7
Sequence was last modified on March 29, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 68)
Name and origin of the protein
DescriptionRecName: Full=ATP-dependent protease ATPase subunit HslU; AltName: Full=Heat shock protein HslU; AltName: Full=Unfoldase HslU;
Gene nameName=hslU
Synonyms=htpI
OrderedLocusNames=b3931, JW3902
Encoded onName=hslU; Synonyms=htpI; OrderedLocusNames=b3931, JW3902
Keywords3D-structure; ATP-binding; Chaperone; Complete proteome; Cytoplasm; Nucleotide-binding; Reference proteome; Stress response.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLL19201; AAB03063.1; -; Genomic_DNA
EMBLU00096; AAC76913.1; -; Genomic_DNA
EMBLAP009048; BAE77379.1; -; Genomic_DNA
PIRJT0761; JT0761; .
RefSeqNP_418366.1; NC_000913.2; .
PDB1DO0; X-ray; 3.00 A; A/B/C/D/E/F=2-443
PDB1DO2; X-ray; 4.00 A; A/B/C/D=2-443
PDB1E94; X-ray; 2.80 A; E/F=2-443
PDB1G4A; X-ray; 3.00 A; E/F=1-443
PDB1G4B; X-ray; 7.00 A; E/F/K/L=1-443
PDB1HQY; X-ray; 2.80 A; E/F=2-443
PDB1HT1; X-ray; 2.80 A; E/F/G/I=2-443
PDB1HT2; X-ray; 2.80 A; E/F/G/H=2-443
PDB1YYF; X-ray; 4.16 A; A/B=1-443
PDBsum1DO0; -; .
PDBsum1DO2; -; .
PDBsum1E94; -; .
PDBsum1G4A; -; .
PDBsum1G4B; -; .
PDBsum1HQY; -; .
PDBsum1HT1; -; .
PDBsum1HT2; -; .
PDBsum1YYF; -; .
ProteinModelPortalP0A6H5; -; .
SMRP0A6H5; 1-443; .
DIPDIP-31855N; -; .
IntActP0A6H5; 48; .
SWISS-2DPAGEP0A6H5; -; .
ECO2DBASED048.5; 6TH EDITION; .
EnsemblBacteriaEBESCT00000003987; EBESCP00000003987; EBESCG00000003262; .
EnsemblBacteriaEBESCT00000018172; EBESCP00000017463; EBESCG00000017227; .
GeneID948430; -; .
GenomeReviewsAP009048_GR; JW3902; .
GenomeReviewsU00096_GR; b3931; .
KEGGecj:JW3902; -; .
KEGGeco:b3931; -; .
EchoBASEEB1827; -; .
EcoGeneEG11881; hslU; .
eggNOGCOG1220; -; .
GeneTreeEBGT00050000010205; -; .
HOGENOMHBG745965; -; .
OMAEASKFTE; -; .
ProtClustDBPRK05201; -; .
BioCycEcoCyc:EG11881-MONOMER; -; .
GenevestigatorP0A6H5; -; .
GOGO:0009376; C:HslUV protease complex; IEA:InterPro; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0016887; F:ATPase activity; IEA:InterPro; .
GOGO:0070011; F:peptidase activity; acting on L-amino acid peptides; IEA:InterPro
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0009408; P:response to heat; IEP:EcoliWiki; .
HAMAPMF_00249; HslU; 1; -
InterProIPR003593; ATPase_AAA+_core; .
InterProIPR013093; ATPase_AAA-2; .
InterProIPR003959; ATPase_AAA_core; .
InterProIPR019489; Clp_ATPase_C; .
InterProIPR004491; HslU; .
PANTHERPTHR11262:SF3; HslU; 1; .
PfamPF00004; AAA; 1; .
PfamPF07724; AAA_2; 1; .
PfamPF10431; ClpB_D2-small; 1; .
SMARTSM00382; AAA; 1; .
TIGRFAMsTIGR00390; HslU; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server