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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0A6M8 |
SWISS-2DPAGE: P0A6M8
P0A6M8:
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ID EFG_ECOLI; STANDARD; 2DG.
AC P0A6M8; P02996;
DT 01-OCT-1998, integrated into SWISS-2DPAGE (release 8).
DT 15-MAY-2003, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 12.
DE RecName: Full=Elongation factor G; Short=EF-G;.
GN Name=fusA; Synonyms=far, fus; OrderedLocusNames=b3340, JW3302;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [2]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0014R2=5.21/91354;
2D -!- PEPTIDE MASSES: SPOT 2D-0014R2: 1597.618; 1732.64; 1820.684;
2D 2067.759; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001L4G=5.23/50424; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001L4G: 902.487; 1045.588; 1089.561;
2D 1098.579; 1112.585; 1254.745; 1414.815; 1515.862; 1531.868; 1732.929;
2D 1820.996; 1951.997; 2059.074; 2062.139; 2078.124; 2101.098; 2201.174;
2D 2217.135; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WCU=5.29/85046;
2D -!- PI/MW: SPOT 2D-001WCV=5.20/84325;
2D -!- PI/MW: SPOT 2D-001WK8=5.51/46525; !FRAGMENT!
2D -!- PI/MW: SPOT 2D-001WN8=5.20/38691; !FRAGMENT!
2D -!- PI/MW: SPOT 2D-001WR8=5.78/32101; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001WCU: 1191.57; 1414.72; 1701.8; 1732.78;
2D 1820.89; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WCV: 1098.52; 1105.49; 1191.65; 1254.74;
2D 1414.82; 1451.76; 1531.85; 1597.9; 1732.92; 1737.94; 1820.98; 1963.07;
2D 2058.99; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WK8: (R)EFNVEANVGKPQVAYR(E),475-490.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WN8: (R)EFNVEANVGKPQVAYR(E),475-490.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WR8: (R)EFNVEANVGKPQVAYR(E),475-490;
2D (K)GGVIPGEYIPAVDK(G),541-554; (K)GQESEVTGVK(I),643-652.
2D -!- MAPPING: SPOTS 1WC*: Peptide mass fingerprinting [2]; SPOTS 1WK8,
2D 1WN8, 1WR8: Tandem mass spectrometry [2].
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DR 2DBase-Ecoli; P0A6M8; EFG_ECOLI.
DR ECO2DBASE; D084.0; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A6M8; EFG_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli(4.5-5.5)
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Escherichia coli(5-6)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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