|
|
|
|
|
Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0A6P1 |
SWISS-2DPAGE: P0A6P1
P0A6P1:
Nice View - a user-friendly view of this entry
ID EFTS_ECOLI; STANDARD; 2DG.
AC P0A6P1; P02997;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 31-MAR-2004, 2D annotation version 6.
DT 19-MAY-2011, general annotation version 15.
DE RecName: Full=Elongation factor Ts; Short=EF-Ts;.
GN Name=tsf; OrderedLocusNames=b0170, JW0165;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
RA Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT ''''''Protein identifications of O'Farrell two-dimensional gels:
RT locations of 81 Escherichia coli proteins'';'';'';
RL J. Bacteriol. 141(1):1409-1420(1980).
RN [4]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [5]
RP PROTEIN EXPRESSION.
RX MEDLINE=79216190; PubMed=156716; [NCBI, Expasy, EBI, Israel, Japan]
RA Herendeen S.L., Vanbogelen R.A., Neidhardt F.C.;
RT ''''''Levels of major proteins of Escherichia coli during growth at
RT different temperatures'';'';'';
RL J. Bacteriol. 139(1):185-194(1979).
RN [6]
RP PROTEIN EXPRESSION.
RX MEDLINE=78212912; PubMed=352533; [NCBI, Expasy, EBI, Israel, Japan]
RA Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.;
RT ''''''Patterns of protein synthesis in E. coli: a catalog of the amount
RT of 140 individual proteins at different growth rates'';'';'';
RL Cell 14(1):179-190(1978).
RN [7]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [8]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000L7K=5.16/33686;
2D -!- PI/MW: SPOT 2D-000L7M=5.06/33758;
2D -!- AMINO ACID COMPOSITION: SPOT 2D-000L7M: B=8.4, Z=11.9, S=4.5,
2D H=1.3, G=9.6, T=4.4, A=12.8, P=2.8, Y=1.7, R=4.3, V=9.9, M=2.6, I=6.5,
2D L=7.5, F=4.7, K=7.3;
2D -!- EXPRESSION: CHANGED VERY LITTLE THROUGHOUT THE NORMAL ARRHENIUS
2D RANGE (23 TO 37°C) [5]; INCREASED IN LEVEL WITH INCREASING GROWTH RATE [6].
2D -!- MAPPING: SPOT 2D-000L7K: AMINO ACID COMPOSITION AND MICROSEQUENCE
2D ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3];
2D SPOT 2D-000L7M: AMINO ACID COMPOSITION AND SEQUENCE TAG (AE) [4].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-00156V=5.22/32533;
2D -!- PEPTIDE MASSES: SPOT 2D-00156V: 908.22; 928.33; 1069.27; 1272.38;
2D 1742.56; 1875.56; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [7].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WPB=5.14/34596;
2D -!- PI/MW: SPOT 2D-001WPF=5.08/34633;
2D -!- PI/MW: SPOT 2D-001WPG=5.20/34415;
2D -!- PEPTIDE MASSES: SPOT 2D-001WPB: 908.34; 928.47; 1069.4; 1084.53;
2D 1272.52; 1742.73; 1898.8; 2268.91; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WPG: 860.39; 908.33; 916.45; 928.47;
2D 1007.41; 1014.47; 1069.39; 1084.53; 1142.55; 1272.53; 1742.74; 1859.75;
2D 1893.79; 1898.81; 1987.82; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WPB: (K)AQFEEER(V),112-118.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WPF: (K)DAGFQAFADKVLDAAVAGK(I),85-103.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WPG: (K)DAGFQAFADK(V),85-94;
2D (K)AQFEEER(V),112-118; (K)IGENINIR(R),125-132.
2D -!- EXPRESSION: increase after benzoic acid treatment [8].
2D -!- MAPPING: SPOTS 1WPB, 1WPG: PEPTIDE MASS FINGERPRINTING [8]; Tandem
2D mass spectrometry [8].
CC ---------------------------------------------------------------------------
CC This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC There are no restrictions on its use by non-profit institutions as long as
CC its content is in no way modified and this statement is not removed.
CC Usage by and for commercial entities requires a license agreement (See
CC http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC ---------------------------------------------------------------------------
DR 2DBase-Ecoli; P0A6P1; EFTS_ECOLI.
DR ECO2DBASE; C030.7; 6TH EDITION.
DR ECO2DBASE; C031.6; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A6P1; EFTS_ECOLI.
//
The following 2-D maps are available for this protein:
|
Escherichia coli
 |
Escherichia coli(4.5-5.5)
 |
Escherichia coli DIGE (4.5-6.5)
 |
|---|
SWISS-2DPAGE (search AC)
|
