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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P0A6Y8 |
SWISS-2DPAGE: P0A6Y8
P0A6Y8:
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ID DNAK_ECOLI; STANDARD; 2DG.
AC P0A6Y8; P04475;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 6.
DT 19-MAY-2011, general annotation version 13.
DE RecName: Full=Chaperone protein DnaK; AltName: Full=HSP70; AltName:
DE Full=Heat shock 70 kDa protein; AltName: Full=Heat shock protein 70;.
GN Name=dnaK; Synonyms=groP, grpF, seg; OrderedLocusNames=b0014, JW0013;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RX MEDLINE=83270974; PubMed=6308410; [NCBI, Expasy, EBI, Israel, Japan]
RA Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.;
RT ''''''Gene-protein index of Escherichia coli K-12'';'';'';
RL Microbiol. Rev. 47(1):231-284(1983).
RN [3]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [4]
RP PROTEIN EXPRESSION.
RX MEDLINE=94042879; PubMed=8226654; [NCBI, Expasy, EBI, Israel, Japan]
RA Gage D.J., Neidhardt F.C.;
RT ''''''Adaptation of Escherichia coli to the uncoupler of oxidative
RT phosphorylation 2,4-dinitrophenol'';'';'';
RL J. Bacteriol. 175(1):7105-7108(1993).
RN [5]
RP PROTEIN EXPRESSION.
RX MEDLINE=87083376; PubMed=3539918; [NCBI, Expasy, EBI, Israel, Japan]
RA Vanbogelen R.A., Kelley P.M., Neidhardt F.C.;
RT ''''''Differential induction of heat shock, SOS, and oxidation stress
RT regulons and accumulation of nucleotides in Escherichia coli'';'';'';
RL J. Bacteriol. 169(1):26-32(1987).
RN [6]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [7]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KIM=4.80/69647;
2D -!- EXPRESSION: INCREASED FOLLOWING EXPOSURE TO DNP [4]; WAS INDUCED BY
2D TEMPERATURE SHIFTS, ETHANOL, NALIDIXIC ACID AND PUROMYCIN [5].
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT
2D [2] AND VANBOGELEN [3].
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HF6=4.98/70671;
2D -!- PI/MW: SPOT 2D-001HFC=4.98/67488;
2D -!- PI/MW: SPOT 2D-001HFS=4.96/61267;
2D -!- PI/MW: SPOT 2D-001HFT=4.97/61125;
2D -!- PI/MW: SPOT 2D-001HFV=4.95/60700;
2D -!- PI/MW: SPOT 2D-001HFW=4.95/60559;
2D -!- PI/MW: SPOT 2D-001HFX=4.96/60841;
2D -!- PI/MW: SPOT 2D-001HFY=4.95/60700;
2D -!- PI/MW: SPOT 2D-001HFZ=4.48/59582;
2D -!- PI/MW: SPOT 2D-001HH6=4.93/52435;
2D -!- PI/MW: SPOT 2D-001HIJ=5.00/44877;
2D -!- PEPTIDE MASSES: SPOT 2D-001HF6: 960.426; 1050.465; 1061.464;
2D 1149.622; 1179.618; 1206.577; 1277.649; 1290.625; 1395.785; 1485.839;
2D 1598.843; 1659.873; 1816.005; 2346.269; 2423.302; 2441.293; 2532.246;
2D 2653.302; 2869.353; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HFC: 960.411; 1050.447; 1149.595;
2D 1179.6; 1206.555; 1267.677; 1277.607; 1286.662; 1290.585; 1395.761;
2D 1485.812; 1500.727; 1598.828; 1659.867; 1815.982; 2346.221; 2423.265;
2D 2441.28; 2532.252; 2653.281; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HFS: 1003.523; 1021.412; 1149.593;
2D 1179.583; 1201.628; 1206.499; 1277.664; 1286.622; 1485.845; 1598.815;
2D 2281.059; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HFT: 1050.487; 1149.638; 1179.593;
2D 1206.582; 1277.671; 1395.742; 1485.806; 1500.777; 1598.838; 2281.021;
2D 2441.302; 2653.295; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HFV: 960.496; 1050.494; 1061.45;
2D 1149.622; 1179.621; 1206.593; 1277.647; 1286.673; 1395.79; 1485.829;
2D 1500.788; 1598.84; 1659.903; 1816.009; 2423.278; 2441.29; 2653.297; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HFW: 944.482; 952.459; 960.461;
2D 1021.532; 1050.497; 1149.62; 1179.593; 1206.57; 1267.695; 1277.64;
2D 1286.676; 1299.647; 1395.759; 1427.797; 1485.804; 1500.79; 1598.804;
2D 1659.865; 1815.939; 1960.963; 2280.975; 2372.132; 2423.22; 2441.23;
2D 2532.193; 2640.16; 2653.203; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HFY: 875.371; 1050.479; 1149.603;
2D 1179.61; 1201.642; 1206.584; 1277.66; 1485.821; 1598.836; 2281.105;
2D 2441.288; 2653.333; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HFZ: 960.401; 1149.611; 1179.596;
2D 1206.551; 1395.789; 1485.793; 1598.835; 2532.328; 2653.348; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HH6: 1149.609; 1179.6; 1395.809;
2D 1485.872; 1598.895; 1816.08; 2441.441; 2653.489; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HIJ: 1050.475; 1179.617; 1277.693;
2D 1485.839; 2441.327; 2653.326; 2869.412; TRYPSIN.
2D -!- MAPPING: SPOTS 1HFT, 1HFV, 1HFW, 1HF6, 1HFC, 1HFS, 1HFY, 1HFZ,
2D 1HH6, 1HIJ: Peptide mass fingerprinting [6]; SPOT 2D-001HFW:
2D MICROSEQUENCING (XFQDE) [6]; SPOTS 1HFW, 1HFX, 1HH6: GEL MATCHING WITH
2D MASTER ECOLI4.5-5.5 [6].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0014TQ=4.96/60487;
2D -!- PI/MW: SPOT 2D-0014TV=4.97/60010;
2D -!- PI/MW: SPOT 2D-0014VD=4.95/53154;
2D -!- PEPTIDE MASSES: SPOT 2D-0014TQ: 861.01; 1149.5; 1206.45; 1277.49;
2D 1286.54; 1598.63; 2280.74; 2440.96; 2531.33; 2652.91; 2868.93; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-0014TV: 875.32; 1149.59; 1206.54; 1277.6;
2D 1286.66; 1598.74; 2280.85; 2441.09; 2653.06; 2869.14; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-0014VD: 1179.64; 1485.85; 1598.87; 1816.01;
2D 2441.29; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [6]; SPOT 2D-0014TQ:
2D MICROSEQUENCING (XFQD) [6].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WDV=4.92/74532;
2D -!- PI/MW: SPOT 2D-001WDY=4.91/74850;
2D -!- PI/MW: SPOT 2D-001WE2=4.94/73587;
2D -!- PEPTIDE MASSES: SPOT 2D-001WDV: 952.45; 960.45; 1050.48; 1149.62;
2D 1206.59; 1299.71; 1395.75; 1427.84; 1544.84; 1598.83; 1659.89; 2190.12; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WE2: 925.49; 952.45; 960.43; 1002.47;
2D 1050.47; 1061.45; 1149.66; 1177.59; 1206.62; 1267.71; 1277.68; 1286.73;
2D 1290.62; 1299.7; 1395.84; 1427.82; 1509.7; 1544.9; 1598.89; 1659.93;
2D 2190.14; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WDY:
2D (K)TAEDYLGEPVTEAVITVPAYFNDAQR,125-150; (R)IINEPTAAALAYGLDKGTGNR(T),167-187.
2D -!- EXPRESSION: increase after benzoic acid treatment [7].
2D -!- MAPPING: SPOTS 1WDV, 1WE2: Peptide mass fingerprinting [7]; SPOT
2D 2D-001WDY: Tandem mass spectrometry [7].
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DR 2DBase-Ecoli; P0A6Y8; DNAK_ECOLI.
DR ECO2DBASE; B066.0; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A6Y8; DNAK_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4-5)
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Escherichia coli(4.5-5.5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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