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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0A799 |
SWISS-2DPAGE: P0A799
P0A799:
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ID PGK_ECOLI; STANDARD; 2DG.
AC P0A799; P11665;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 5.
DT 19-MAY-2011, general annotation version 13.
DE RecName: Full=Phosphoglycerate kinase; EC=2.7.2.3;.
GN Name=pgk; OrderedLocusNames=b2926, JW2893;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [5]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: MONOMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KZ9=5.07/42043;
2D -!- PI/MW: SPOT 2D-000KZD=5.02/41877;
2D -!- PI/MW: SPOT 2D-000KZS=5.00/41546;
2D -!- AMINO ACID COMPOSITION: SPOT 2D-000KZD: B=9, Z=8.5, S=5.7, H=1.1,
2D G=9.3, T=5.6, A=11.4, P=4.5, Y=2.5, R=4.5, V=8.8, M=1.6, I=6.1, L=10.1,
2D F=4.4, K=6.9;
2D -!- MAPPING: SPOT 2D-000KZ9: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED
2D ON 2-D GELS BY VANBOGELEN [2]; SPOTS 2D-000KZD, 2D-000KZS: AMINO ACID
2D COMPOSITION AND SEQUENCE TAG (SVIK) [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-00151K=5.15/40464;
2D -!- PI/MW: SPOT 2D-00152G=5.14/39816;
2D -!- PEPTIDE MASSES: SPOT 2D-00151K: 1243.668; 1427.773; 1464.752;
2D 1508.787; 1584.833; 1739.978; 1874.967; 1993.063; 2204.12; 2242.149;
2D 2465.358; TRYPSIN.
2D -!- MAPPING: MICROSEQUENCING (XVIK AND XVFI) [4]; SPOT 2D-00151K: MASS
2D FINGERPRINTING [4].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WI6=5.30/52681;
2D -!- PEPTIDE MASSES: SPOT 2D-001WI6: 928.45; 969.37; 975.43; 1119.43;
2D 1155.47; 1227.5; 1352.47; 1411.52; 1424.63; 1426.6; 1464.51; 1508.52;
2D 1584.58; 1723.68; 1874.59; 1992.65; 2043.57; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WI6: (R)ASLPTIELALK(Q),38-48;
2D (K)YAALCDVFVMDAFGTAHR(A),128-145; (R)AQASTHGIGK(F),146-155;
2D (K)FADVACAGPLLAAELDALGK(A),156-175; (K)LTVLDSLSK(I),197-205;
2D (K)IADQLIVGGGIANTFIAAQGHDVGK(S),206-230; (R)LLTTCNIPVPSDVR(V),244-257;
2D (K)TILWNGPVGVFEFPNFR(K),302-318; (K)VLPAVAMLEER(A),373-383.
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [5].
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DR 2DBase-Ecoli; P0A799; PGK_ECOLI.
DR UniProtKB/Swiss-Prot; P0A799; PGK_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4.5-5.5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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