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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A7E1




SWISS-2DPAGE:  P0A7E1


P0A7E1


General information about the entry
View entry in simple text format
Entry namePYRD_ECOLI
Primary accession numberP0A7E1
Secondary accession number(s) P05021
integrated into SWISS-2DPAGE on December 1, 2000 (release 13)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on November 9, 2011 (version 8)
Name and origin of the protein
DescriptionRecName: Full=Dihydroorotate dehydrogenase (quinone); EC=1.3.5.2; AltName: Full=DHOdehase; Short=DHOD; Short=DHODase; AltName: Full=Dihydroorotate oxidase;.
Gene nameName=pyrD
OrderedLocusNames=b0945, JW0928
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI6-11 {Escherichia coli(6-11)}
Escherichia coli
ECOLI6-11
  map experimental info
  protein estimated location
 
ECOLI6-11

MAP LOCATIONS:
pI=7.28; Mw=47786  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP0A7E1; PYRD_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePYRD_ECOLI
Primary accession numberP0A7E1
Secondary accession number(s) P05021
Sequence was last modified on August 13, 1987 (version 1)
Annotations were last modified on October 19, 2011 (version 69)
Name and origin of the protein
DescriptionRecName: Full=Dihydroorotate dehydrogenase (quinone); EC=1.3.5.2; AltName: Full=DHOdehase; Short=DHOD; Short=DHODase; AltName: Full=Dihydroorotate oxidase;
Gene nameName=pyrD
OrderedLocusNames=b0945, JW0928
Encoded onName=pyrD; OrderedLocusNames=b0945, JW0928
Keywords3D-structure; Cell membrane; Complete proteome; Direct protein sequencing; Flavoprotein; FMN; Membrane; Oxidoreductase; Pyrimidine biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX02826; CAA26594.1; -; Genomic_DNA
EMBLU00096; AAC74031.1; -; Genomic_DNA
EMBLAP009048; BAA35700.1; -; Genomic_DNA
PIRA23109; DEECDO; .
RefSeqNP_415465.1; NC_000913.2; .
PDB1F76; X-ray; 2.50 A; A/B/D/E=1-336
PDBsum1F76; -; .
ProteinModelPortalP0A7E1; -; .
SMRP0A7E1; 1-336; .
DIPDIP-35945N; -; .
IntActP0A7E1; 6; .
SWISS-2DPAGEP0A7E1; -; .
PRIDEP0A7E1; -; .
EnsemblBacteriaEBESCT00000005052; EBESCP00000005052; EBESCG00000004123; .
EnsemblBacteriaEBESCT00000014954; EBESCP00000014245; EBESCG00000014014; .
GeneID945556; -; .
GenomeReviewsAP009048_GR; JW0928; .
GenomeReviewsU00096_GR; b0945; .
KEGGecj:JW0928; -; .
KEGGeco:b0945; -; .
EchoBASEEB0800; -; .
EcoGeneEG10807; pyrD; .
eggNOGCOG0167; -; .
GeneTreeEBGT00050000011106; -; .
HOGENOMHBG351027; -; .
OMASYVTVNI; -; .
ProtClustDBPRK05286; -; .
BioCycEcoCyc:DIHYDROOROTOX-MONOMER; -; .
BioCycMetaCyc:DIHYDROOROTOX-MONOMER; -; .
GenevestigatorP0A7E1; -; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0004158; F:dihydroorotate oxidase activity; IEA:InterPro; .
GOGO:0006207; P:'de novo' pyrimidine base biosynthetic process; IEA:InterPro; .
GOGO:0019877; P:diaminopimelate biosynthetic process; IDA:EcoliWiki; .
GOGO:0006222; P:UMP biosynthetic process; IEA:InterPro; .
HAMAPMF_00225; DHO_dh_type2; 1; -
InterProIPR013785; Aldolase_TIM; .
InterProIPR012135; Dihydroorotate_DH_1_2; .
InterProIPR005719; Dihydroorotate_DH_2; .
InterProIPR001295; Dihydroorotate_DH_CS; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
PfamPF01180; DHO_dh; 1; .
PIRSFPIRSF000164; DHO_oxidase; 1; .
TIGRFAMsTIGR01036; PyrD_sub2; 1; .
PROSITEPS00911; DHODEHASE_1; 1; .
PROSITEPS00912; DHODEHASE_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server