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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A7F3




SWISS-2DPAGE:  P0A7F3


P0A7F3


General information about the entry
View entry in simple text format
Entry namePYRI_ECOLI
Primary accession numberP0A7F3
Secondary accession number(s) P00478
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Aspartate carbamoyltransferase regulatory chain;.
Gene nameName=pyrI
OrderedLocusNames=b4244, JW4203
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
MEDLINE=83270974; PubMed=6308410; [NCBI, ExPASy, EBI, Israel, Japan]
Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.
''''''Gene-protein index of Escherichia coli K-12'';'';''
Microbiol. Rev. 47(1):231-284(1983)
[3]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
Comments
  • SUBUNIT: CONTAINS SIX CATALYTIC AND SIX REGULATORY CHAINS
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=6.84; Mw=16989

MAPPING (identification):
AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT [2] AND VANBOGELEN [3].



ECOLI6-11 {Escherichia coli(6-11)}
Escherichia coli
ECOLI6-11
  map experimental info
  protein estimated location
 
ECOLI6-11

MAP LOCATIONS:
pI=7.01; Mw=17642  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEI017.5; 6TH EDITION.
UniProtKB/Swiss-ProtP0A7F3; PYRI_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePYRI_ECOLI
Primary accession numberP0A7F3
Secondary accession number(s) P00478 Q2M663
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 66)
Name and origin of the protein
DescriptionRecName: Full=Aspartate carbamoyltransferase regulatory chain;
Gene nameName=pyrI
OrderedLocusNames=b4244, JW4203
Encoded onName=pyrI; OrderedLocusNames=b4244, JW4203
Keywords3D-structure; Complete proteome; Direct protein sequencing; Metal-binding; Pyrimidine biosynthesis; Reference proteome; Zinc.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLK01472; AAA24477.1; -; Genomic_DNA
EMBLU14003; AAA97141.1; -; Genomic_DNA
EMBLU00096; AAC77201.1; -; Genomic_DNA
EMBLAP009048; BAE78243.1; -; Genomic_DNA
EMBLM28578; AAA24487.1; -; Genomic_DNA
PIRA93985; DTECR; .
RefSeqNP_418665.1; NC_000913.2; .
PDB1ACM; X-ray; 2.80 A; B/D=2-152
PDB1AT1; X-ray; 2.80 A; B/D=2-152
PDB1D09; X-ray; 2.10 A; B/D=1-153
PDB1EZZ; X-ray; 2.70 A; B/D=1-153
PDB1F1B; X-ray; 2.30 A; B/D=1-153
PDB1I5O; X-ray; 2.80 A; B/D=1-153
PDB1NBE; X-ray; 2.60 A; B/D=2-152
PDB1Q95; X-ray; 2.46 A; G/H/I/J/K/L=1-152
PDB1R0B; X-ray; 2.90 A; G/H/I/J/K/L=1-152
PDB1R0C; X-ray; 2.37 A; B/H=1-152
PDB1RAA; X-ray; 2.50 A; B/D=2-152
PDB1RAB; X-ray; 2.50 A; B/D=2-152
PDB1RAC; X-ray; 2.50 A; B/D=2-152
PDB1RAD; X-ray; 2.50 A; B/D=2-152
PDB1RAE; X-ray; 2.50 A; B/D=2-152
PDB1RAF; X-ray; 2.50 A; B/D=2-152
PDB1RAG; X-ray; 2.50 A; B/D=2-152
PDB1RAH; X-ray; 2.50 A; B/D=2-152
PDB1RAI; X-ray; 2.50 A; B/D=2-152
PDB1SKU; X-ray; 2.60 A; B/D=1-152
PDB1TTH; X-ray; 2.80 A; B/D=2-152
PDB1TU0; X-ray; 2.55 A; B/D=2-152
PDB1TUG; X-ray; 2.10 A; B/D=2-152
PDB1XJW; X-ray; 2.71 A; B/D=2-152
PDB1ZA1; X-ray; 2.20 A; B/D=1-153
PDB1ZA2; X-ray; 2.50 A; B/D=1-153
PDB2A0F; X-ray; 2.90 A; B/D=2-152
PDB2AIR; X-ray; 2.00 A; B/H=1-153
PDB2AT1; X-ray; 2.80 A; B/D=2-152
PDB2ATC; X-ray; 3.00 A; B=6-152
PDB2FZC; X-ray; 2.10 A; B/D=1-152
PDB2FZG; X-ray; 2.25 A; B/D=1-152
PDB2FZK; X-ray; 2.50 A; B/D=1-152
PDB2H3E; X-ray; 2.30 A; B/D=1-152
PDB2HSE; X-ray; 2.60 A; B/D=2-152
PDB2IPO; X-ray; 2.60 A; B/D=2-152
PDB2QG9; X-ray; 2.70 A; B/D=1-153
PDB2QGF; X-ray; 2.20 A; B/D=1-153
PDB3AT1; X-ray; 2.80 A; B/D=2-152
PDB3D7S; X-ray; 2.80 A; B/D=1-153
PDB3MPU; X-ray; 2.86 A; B/D/F=1-153
PDB4AT1; X-ray; 2.60 A; B/D=1-153
PDB5AT1; X-ray; 2.60 A; B/D=1-153
PDB6AT1; X-ray; 2.60 A; B/D=1-153
PDB7AT1; X-ray; 2.80 A; B/D=1-153
PDB8AT1; X-ray; 2.80 A; B/D=1-153
PDB8ATC; X-ray; 2.50 A; B/D=2-152
PDB9ATC; X-ray; 2.40 A; B=8-152
PDBsum1ACM; -; .
PDBsum1AT1; -; .
PDBsum1D09; -; .
PDBsum1EZZ; -; .
PDBsum1F1B; -; .
PDBsum1I5O; -; .
PDBsum1NBE; -; .
PDBsum1Q95; -; .
PDBsum1R0B; -; .
PDBsum1R0C; -; .
PDBsum1RAA; -; .
PDBsum1RAB; -; .
PDBsum1RAC; -; .
PDBsum1RAD; -; .
PDBsum1RAE; -; .
PDBsum1RAF; -; .
PDBsum1RAG; -; .
PDBsum1RAH; -; .
PDBsum1RAI; -; .
PDBsum1SKU; -; .
PDBsum1TTH; -; .
PDBsum1TU0; -; .
PDBsum1TUG; -; .
PDBsum1XJW; -; .
PDBsum1ZA1; -; .
PDBsum1ZA2; -; .
PDBsum2A0F; -; .
PDBsum2AIR; -; .
PDBsum2AT1; -; .
PDBsum2ATC; -; .
PDBsum2FZC; -; .
PDBsum2FZG; -; .
PDBsum2FZK; -; .
PDBsum2H3E; -; .
PDBsum2HSE; -; .
PDBsum2IPO; -; .
PDBsum2QG9; -; .
PDBsum2QGF; -; .
PDBsum3AT1; -; .
PDBsum3D7S; -; .
PDBsum3MPU; -; .
PDBsum4AT1; -; .
PDBsum5AT1; -; .
PDBsum6AT1; -; .
PDBsum7AT1; -; .
PDBsum8AT1; -; .
PDBsum8ATC; -; .
PDBsum9ATC; -; .
ProteinModelPortalP0A7F3; -; .
SMRP0A7F3; 1-153; .
IntActP0A7F3; 2; .
SWISS-2DPAGEP0A7F3; -; .
ECO2DBASEI017.5; 6TH EDITION; .
EnsemblBacteriaEBESCT00000000693; EBESCP00000000693; EBESCG00000000580; .
EnsemblBacteriaEBESCT00000000694; EBESCP00000000694; EBESCG00000000580; .
EnsemblBacteriaEBESCT00000017573; EBESCP00000016864; EBESCG00000016629; .
GeneID948763; -; .
GenomeReviewsAP009048_GR; JW4203; .
GenomeReviewsU00096_GR; b4244; .
KEGGecj:JW4203; -; .
KEGGeco:b4244; -; .
EchoBASEEB0804; -; .
EcoGeneEG10811; pyrI; .
eggNOGCOG1781; -; .
GeneTreeEBGT00050000011774; -; .
HOGENOMHBG305327; -; .
OMATVIDHIT; -; .
ProtClustDBPRK00893; -; .
BioCycEcoCyc:ASPCARBREG-MONOMER; -; .
BioCycMetaCyc:ASPCARBREG-MONOMER; -; .
GenevestigatorP0A7F3; -; .
GOGO:0009347; C:aspartate carbamoyltransferase complex; IEA:InterPro; .
GOGO:0005737; C:cytoplasm; IDA:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0008270; F:zinc ion binding; IDA:EcoCyc; .
GOGO:0006207; P:'de novo' pyrimidine base biosynthetic process; IEA:InterPro; .
GOGO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:UniProtKB-KW; .
HAMAPMF_00002; Asp_carb_tr_reg; 1; -
InterProIPR020545; Asp_carbamoyltransf_reg_N; .
InterProIPR002801; Asp_carbamoylTrfase_reg; .
InterProIPR020542; Asp_carbamoyltrfase_reg_C; .
Gene3DG3DSA:2.30.30.20; PyrI; 1; .
Gene3DG3DSA:3.30.70.140; PyrI; 1; .
PfamPF01948; PyrI; 1; .
PfamPF02748; PyrI_C; 1; .
ProDomPD006194; Asp_carbamoylTrfase_reg; 1; .
SUPFAMSSF54893; Asp_carbamoylTrfase_reg; 1; .
SUPFAMSSF57825; Asp_transf_reg_C; 1; .
TIGRFAMsTIGR00240; ATCase_reg; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server