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Sample Preparation and Post-separation Analysis

P0A817:

ID   METK_ECOLI; STANDARD; 2DG.
AC   P0A817; P04384;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 19.
DE   RecName: Full=S-adenosylmethionine synthase; Short=AdoMet synthase;
DE   EC=2.5.1.6; AltName: Full=MAT; AltName: Full=Methionine adenosyltransferase;.
GN   Name=metK; Synonyms=metX; OrderedLocusNames=b2942, JW2909;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4.5-5.5.
IM   ECOLI, ECOLI4.5-5.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
RA   Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT   ''''''Protein identifications of O'Farrell two-dimensional gels:
RT   locations of 81 Escherichia coli proteins'';'';'';
RL   J. Bacteriol. 141(1):1409-1420(1980).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
CC   -!- SUBUNIT: HOMOTETRAMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KWM=5.03/45059;
2D   -!-   MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D         [2] AND BLOCH [3].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-0014ZS=5.13/43079;
2D   -!-   PI/MW: SPOT 2D-00158A=5.00/30312;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0014ZS: 951.309; 999.354; 1108.284;
2D         1211.364; 1254.499; 1339.441; 1367.48; 1552.442; 1669.49; 2053.726;
2D         2180.754; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-00158A: 951.494; 1211.59; 1228.59; 1287.61;
2D         1552.7; 1669.79; 2054.05; 2181.15; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
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DR   2DBase-Ecoli; P0A817; METK_ECOLI.
DR   ECO2DBASE; C044.6; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A817; METK_ECOLI.
//

The following 2-D maps are available for this protein:

	Escherichia coli	Escherichia coli(4.5-5.5)

SWISS-2DPAGE (search AC)