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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P0A817 |
SWISS-2DPAGE: P0A817
P0A817:
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ID METK_ECOLI; STANDARD; 2DG.
AC P0A817; P04384;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 31-MAR-2004, 2D annotation version 2.
DT 19-MAY-2011, general annotation version 19.
DE RecName: Full=S-adenosylmethionine synthase; Short=AdoMet synthase;
DE EC=2.5.1.6; AltName: Full=MAT; AltName: Full=Methionine adenosyltransferase;.
GN Name=metK; Synonyms=metX; OrderedLocusNames=b2942, JW2909;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4.5-5.5.
IM ECOLI, ECOLI4.5-5.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
RA Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT ''''''Protein identifications of O'Farrell two-dimensional gels:
RT locations of 81 Escherichia coli proteins'';'';'';
RL J. Bacteriol. 141(1):1409-1420(1980).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
CC -!- SUBUNIT: HOMOTETRAMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KWM=5.03/45059;
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D [2] AND BLOCH [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0014ZS=5.13/43079;
2D -!- PI/MW: SPOT 2D-00158A=5.00/30312;
2D -!- PEPTIDE MASSES: SPOT 2D-0014ZS: 951.309; 999.354; 1108.284;
2D 1211.364; 1254.499; 1339.441; 1367.48; 1552.442; 1669.49; 2053.726;
2D 2180.754; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-00158A: 951.494; 1211.59; 1228.59; 1287.61;
2D 1552.7; 1669.79; 2054.05; 2181.15; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
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DR 2DBase-Ecoli; P0A817; METK_ECOLI.
DR ECO2DBASE; C044.6; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A817; METK_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4.5-5.5)
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SWISS-2DPAGE (search AC)
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