World-2DPAGE Home
Home  |  Contact

Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A825




SWISS-2DPAGE: P0A825



P0A825:

Nice View - a user-friendly view of this entry
ID   GLYA_ECOLI; STANDARD; 2DG.
AC   P0A825; P00477;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 14.
DE   RecName: Full=Serine hydroxymethyltransferase; Short=SHMT; Short=Serine
DE   methylase; EC=2.1.2.1;.
GN   Name=glyA; OrderedLocusNames=b2551, JW2535;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: HOMOTETRAMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KVN=5.94/46234;
2D   -!-   PI/MW: SPOT 2D-000KVU=6.04/46051;
2D   -!-   MAPPING: SPOT 2D-000KVN: AMINO ACID COMPOSITION AND SEQUENCE TAG
2D         (MLK) [3]; SPOT 2D-000KVU: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON
2D         2-D GELS BY VANBOGELEN [2].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WKH=6.02/45274;
2D   -!-   PI/MW: SPOT 2D-001WKJ=6.00/45000;
2D   -!-   PI/MW: SPOT 2D-001WLG=6.00/43217;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WKH: 846.45; 963.47; 1040.44; 1109.49;
2D         1320.58; 1478.7; 1814.91; 2065.94; 2163.01; 2480.19; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WKJ: 870.43; 882.36; 963.46; 1010.45;
2D         1040.43; 1093.49; 1927.84; 2015.8; 2162.82; 2318.94; 2441.07; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WKH: (K)GGSEELYK(K),243-250;
2D         (K)TYQQQVAK(N),286-293; (K)NSVPNDPKSPFVTSGIR(V),347-363.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WKJ:
2D         (K)LYNIVPYGIDATGHIDYADLEK(Q),139-160; (K)GGSEELYK(S),243-250;
2D         (K)VVSGGTDNHLFLVDLVDK(N),309-326; (K)NSVPNDPK(S),347-354.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WLG: (K)TYQQQVAK(N),286-293; (K)NSVPNDPKSPFVTSGIR(V),347-363.
2D   -!-   EXPRESSION: increase after benzoic acid treatment [4].
2D   -!-   MAPPING: SPOTS 1WK*: PEPTIDE MASS FINGERPRINTING [4]; Tandem mass
2D         spectrometry [4].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   2DBase-Ecoli; P0A825; GLYA_ECOLI.
DR   ECO2DBASE; G043.8; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A825; GLYA_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server