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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P0A850 |
SWISS-2DPAGE: P0A850
P0A850:
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ID TIG_ECOLI; STANDARD; 2DG.
AC P0A850; P22257;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 5.
DT 19-MAY-2011, general annotation version 11.
DE RecName: Full=Trigger factor; Short=TF;.
GN Name=tig; OrderedLocusNames=b0436, JW0426;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [4]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KRS=4.83/51128;
2D -!- MAPPING: TAGGING ANALYSIS AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HHI=4.96/49707;
2D -!- PI/MW: SPOT 2D-001HHJ=4.58/49476;
2D -!- PI/MW: SPOT 2D-001HHV=4.97/47893;
2D -!- PI/MW: SPOT 2D-001HIE=4.87/45401;
2D -!- PEPTIDE MASSES: SPOT 2D-001HHI: 944.461; 1027.524; 1149.595;
2D 1264.677; 1266.732; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HHJ: 826.422; 993.464; 1027.546;
2D 1106.537; 1251.636; 1264.63; 1266.807; 1289.708; 1458.791; 1576.823;
2D 1583.827; 1886.85; 2039.073; 2439.171; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HHV: 826.427; 944.461; 1027.496;
2D 1149.52; 1264.62; 1266.781; 1428.675; 1445.782; 1583.863; 2039.031; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HIE: 924.386; 933.531; 940.388; 944.504;
2D 993.488; 1014.476; 1106.546; 1122.54; 1133.566; 1149.583; 1204.586;
2D 1251.638; 1264.629; 1266.79; 1289.703; 1420.717; 1422.899; 1445.814;
2D 1576.781; 1583.834; 1719.859; 1833.909; 2039.038; 2195.134; 2439.09;
2D 2829.172; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [3]; SPOT 2D-001HHI: GEL
2D MATCHING WITH MASTER ECOLI4.5-5.5 [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0014WR=4.93/48981;
2D -!- PI/MW: SPOT 2D-0014X1=4.97/48212;
2D -!- PI/MW: SPOT 2D-0014XC=4.95/47081;
2D -!- PEPTIDE MASSES: SPOT 2D-0014WR: 1122.55; 1264.624; 1289.661;
2D 1576.794; 1583.898; 1886.905; 2039.064; 2439.028; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-0014X1: 933.4; 1205.69; 1228.62; 1266.62;
2D 1289.5; 1576.74; 1583.77; 1886.72; 2038.7; 2438.73; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [3].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WI0=4.94/52681;
2D -!- PEPTIDE MASSES: SPOT 2D-001WI0: 856.5; 862.44; 919.46; 933.43;
2D 944.45; 987.5; 1133.46; 1149.46; 1171.59; 1190.53; 1204.5; 1218.44;
2D 1266.69; 1445.66; 1531.69; 1687.81; 1719.72; 1800.81; 1886.72; 2038.87;
2D 2194.95; 2438.98; 2623.96; 2828.98; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WI0: (R)VTITIAADSIETAVK(S),15-29;
2D (R)NFIDAIIK(E),74-81; (R)NFIDAIIKEK(I),74-83; (R)ELPELTAEFIK(R),244-254;
2D (R)FGVEDGSVEGLRAEVR(K),256-271; (K)ANDIDVPAALIDSEIDVLRR(Q),297-316;
2D (R)VVVGLLLGEVIR(T),345-356; (R)NVALEEQAVEAVLAK(A),400-414.
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [4].
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DR ECO2DBASE; B050.3; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A850; TIG_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4-5)
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Escherichia coli(4.5-5.5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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