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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A855




SWISS-2DPAGE:  P0A855


P0A855


General information about the entry
View entry in simple text format
Entry nameTOLB_ECOLI
Primary accession numberP0A855
Secondary accession number(s) P19935
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Protein tolB; Flags: Precursor;.
Gene nameName=tolB
OrderedLocusNames=b0740, JW5100
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.91; Mw=44176  [identification data]
pI=5.98; Mw=43139

MAPPING (identification):
SPOT 2D-000KXL: AMINO ACID COMPOSITION AND MICROSEQUENCING (EVRIVIDSV) [3];
SPOT 2D-000KYE: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI6-11 {Escherichia coli(6-11)}
Escherichia coli
ECOLI6-11
  map experimental info
  protein estimated location
 
ECOLI6-11

MAP LOCATIONS:
pI=7.05; Mw=35331  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0A855; TOLB_ECOLI.
UniProtKB/Swiss-ProtP0A855; TOLB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameTOLB_ECOLI
Primary accession numberP0A855
Secondary accession number(s) P19935
Sequence was last modified on June 7, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 62)
Name and origin of the protein
DescriptionRecName: Full=Protein tolB; Flags: Precursor;
Gene nameName=tolB
OrderedLocusNames=b0740, JW5100
Encoded onName=tolB; OrderedLocusNames=b0740, JW5100
Keywords3D-structure; Bacteriocin transport; Complete proteome; Direct protein sequencing; Periplasm; Protein transport; Reference proteome; Signal; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM28232; AAA24684.1; ALT_INIT; Genomic_DNA
EMBLU00096; AAC73834.1; -; Genomic_DNA
EMBLAP009048; BAA35406.2; -; Genomic_DNA
EMBLX65796; CAA46672.1; -; Genomic_DNA
PIRC64810; C64810; .
RefSeqNP_415268.1; NC_000913.2; .
PDB1C5K; X-ray; 2.00 A; A=1-430
PDB1CRZ; X-ray; 1.95 A; A=28-430
PDB2HQS; X-ray; 1.50 A; A/B/D/F=23-430
PDB2IVZ; X-ray; 2.00 A; A/B/C/D=1-430
PDB2W8B; X-ray; 1.86 A; A/B/D/F=22-430
PDB3IAX; X-ray; 2.60 A; A=1-430
PDBsum1C5K; -; .
PDBsum1CRZ; -; .
PDBsum2HQS; -; .
PDBsum2IVZ; -; .
PDBsum2W8B; -; .
PDBsum3IAX; -; .
ProteinModelPortalP0A855; -; .
SMRP0A855; 23-430; .
DIPDIP-48262N; -; .
TCDB2.C.1.2.1; TonB-ExbB-ExbD/TolA-TolQ-TolR (TonB) family of auxiliary proteins for energization of outer membrane receptor (OMR)-mediated active transport; .
SWISS-2DPAGEP0A855; -; .
2DBase-EcoliP0A855; -; .
EnsemblBacteriaEBESCT00000001423; EBESCP00000001423; EBESCG00000001185; .
EnsemblBacteriaEBESCT00000017030; EBESCP00000016321; EBESCG00000016089; .
GeneID945429; -; .
GenomeReviewsAP009048_GR; JW5100; .
GenomeReviewsU00096_GR; b0740; .
KEGGecj:JW5100; -; .
KEGGeco:b0740; -; .
EchoBASEEB1001; -; .
EcoGeneEG11008; tolB; .
eggNOGCOG0823; -; .
GeneTreeEBGT00050000010794; -; .
HOGENOMHBG644919; -; .
OMARVADYDG; -; .
ProtClustDBPRK03629; -; .
BioCycEcoCyc:EG11008-MONOMER; -; .
GenevestigatorP0A855; -; .
GOGO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoliWiki; .
GOGO:0043213; P:bacteriocin transport; IMP:EcoliWiki; .
GOGO:0017038; P:protein import; IMP:EcoliWiki; .
HAMAPMF_00671; TolB; 1; -
InterProIPR011659; PD40; .
InterProIPR014167; Tol-Pal_propeller_TolB; .
InterProIPR007195; TolB_N; .
InterProIPR015943; WD40/YVTN_repeat-like_dom; .
Gene3DG3DSA:3.40.50.10070; TolB_N; 1; .
Gene3DG3DSA:2.130.10.10; WD40/YVTN_repeat-like; 1; .
PfamPF07676; PD40; 4; .
PfamPF04052; TolB_N; 1; .
SUPFAMSSF52964; TolB_N; 1; .
TIGRFAMsTIGR02800; Propeller_TolB; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server