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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P0A862 |
SWISS-2DPAGE: P0A862
P0A862:
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ID TPX_ECOLI; STANDARD; 2DG.
AC P0A862; P37901;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 15.
DE RecName: Full=Thiol peroxidase; EC=1.11.1.-; AltName: Full=Scavengase P20;.
GN Name=tpx; Synonyms=yzzJ; OrderedLocusNames=b1324, JW1317;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [3]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000LN2=4.89/18709;
2D -!- MAPPING: MICROSEQUENCE ANALYSIS [1].
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HN3=5.02/15095;
2D -!- MAPPING: GEL MATCHING WITH MASTER ECOLI4.5-5.5 [2].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0015DZ=5.00/17510;
2D -!- PEPTIDE MASSES: SPOT 2D-0015DZ: 978.55; 1350.685; 1841.903;
2D 1861.993; 1876.988; 1898.939; 1918.992; 2367.214; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [2].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WWA=4.99/19656;
2D -!- PEPTIDE MASSES: SPOT 2D-001WWA: 978.48; 1350.57; 1506.68; 1876.8;
2D 1898.78; 1918.84; 2046.92; 2345.04; 3804.31; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WWA:
2D (-)SQTVHFQGNPVTVANSIPQAGSK(A),1-23; (K)AQTFTLVAK(D),24-32;
2D (K)DLSDVTLGQFAGK(R),33-45; (R)KVLNIFPSIDTGVCAASVR(K),47-65;
2D (K)VLNIFPSIDTGVCAASVRK(F),48-66; (R)KFNQLATEIDNTVVLCISADLPFAQSR,66-92;
2D (R)FCGAEGLNNVITLSTFR(N),93-109; (R)NAEFLQAYGVAIADGPLK(G),110-127.
2D -!- EXPRESSION: decrease after benzoic acid treatment [3].
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [3].
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DR 2DBase-Ecoli; P0A862; TPX_ECOLI.
DR UniProtKB/Swiss-Prot; P0A862; TPX_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4-5)
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Escherichia coli(4.5-5.5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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