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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0A877 |
SWISS-2DPAGE: P0A877
P0A877:
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ID TRPA_ECOLI; STANDARD; 2DG.
AC P0A877; P00928;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 31-MAR-2004, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 12.
DE RecName: Full=Tryptophan synthase alpha chain; EC=4.2.1.20;.
GN Name=trpA; OrderedLocusNames=b1260, JW1252;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4.5-5.5, ECOLI5-6.
IM ECOLI, ECOLI4.5-5.5, ECOLI5-6.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
RA Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT ''''''Protein identifications of O'Farrell two-dimensional gels:
RT locations of 81 Escherichia coli proteins'';'';'';
RL J. Bacteriol. 141(1):1409-1420(1980).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
CC -!- SUBUNIT: TETRAMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000LER=5.30/28724;
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D [2] AND BLOCH [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-001598=5.38/28936;
2D -!- PI/MW: SPOT 2D-00159R=5.37/27906;
2D -!- PEPTIDE MASSES: SPOT 2D-001598: 972.5028; 1383.691; 1526.614; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-00159R: 874.455; 972.53; 1098.585;
2D 1146.688; 1349.71; 1359.616; 1542.807; 2025.174; 2085.078; 2102.05; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001LGC=5.18/32691;
2D -!- PEPTIDE MASSES: SPOT 2D-001LGC: 874.557; 972.647; 1098.73;
2D 1146.829; 1349.877; 1359.779; 1543.024; 2009.255; 2085.338; 2102.303;
2D 2177.344; 2232.439; 2343.487; 3621.252; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
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DR 2DBase-Ecoli; P0A877; TRPA_ECOLI.
DR ECO2DBASE; E025.5; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A877; TRPA_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4.5-5.5)
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Escherichia coli(5-6)
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SWISS-2DPAGE (search AC)
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