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Sample Preparation and Post-separation Analysis

Searching in 'SWISS-2DPAGE' for entry matching: P0A8G6



General information about the entry
View entry in simple text format
Entry nameWRBA_ECOLI
Primary accession numberP0A8G6
Secondary accession number(s) P30849
integrated into SWISS-2DPAGE on April 1, 2000 (release 12)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 9)
Name and origin of the protein
DescriptionRecName: Full=Flavoprotein wrbA; AltName: Full=Trp repressor-binding protein;.
Gene nameName=wrbA
OrderedLocusNames=b1004, JW0989
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
  map experimental info
  protein estimated location

pI=5.51; Mw=28018  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
  map experimental info
  protein estimated location

pI=5.48; Mw=24005  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2].

This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See or send email from
2DBase-EcoliP0A8G6; WRBA_ECOLI.
UniProtKB/Swiss-ProtP0A8G6; WRBA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameWRBA_ECOLI
Primary accession numberP0A8G6
Secondary accession number(s) P30849 P75890 P77543
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 61)
Name and origin of the protein
DescriptionRecName: Full=Flavoprotein wrbA; AltName: Full=Trp repressor-binding protein;
Gene nameName=wrbA
OrderedLocusNames=b1004, JW0989
Encoded onName=wrbA; OrderedLocusNames=b1004, JW0989
Keywords3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Flavoprotein; FMN; Reference proteome.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLM99166; AAA24759.1; -; Genomic_DNA
EMBLU00096; AAC74089.1; -; Genomic_DNA
EMBLAP009048; BAA35771.1; -; Genomic_DNA
PIRB64842; B64842; .
RefSeqNP_415524.1; NC_000913.2; .
PDB2R96; X-ray; 2.60 A; A/C=1-198
PDB2R97; X-ray; 2.00 A; A/C=1-198
PDB2RG1; X-ray; 1.85 A; A/B=1-198
PDB3B6I; X-ray; 1.66 A; A/B=1-198
PDB3B6J; X-ray; 2.05 A; A/B=1-198
PDB3B6K; X-ray; 1.99 A; A/B=1-198
PDB3B6M; X-ray; 1.85 A; A/B=1-198
PDBsum2R96; -; .
PDBsum2R97; -; .
PDBsum2RG1; -; .
PDBsum3B6I; -; .
PDBsum3B6J; -; .
PDBsum3B6K; -; .
PDBsum3B6M; -; .
ProteinModelPortalP0A8G6; -; .
SMRP0A8G6; 2-198; .
DIPDIP-36231N; -; .
IntActP0A8G6; 3; .
MINTMINT-1272067; -; .
2DBase-EcoliP0A8G6; -; .
EnsemblBacteriaEBESCT00000001097; EBESCP00000001097; EBESCG00000000910; .
EnsemblBacteriaEBESCT00000017523; EBESCP00000016814; EBESCG00000016579; .
GeneID947263; -; .
GenomeReviewsAP009048_GR; JW0989; .
GenomeReviewsU00096_GR; b1004; .
KEGGecj:JW0989; -; .
KEGGeco:b1004; -; .
EchoBASEEB1502; -; .
EcoGeneEG11540; wrbA; .
eggNOGCOG0655; -; .
GeneTreeEBGT00050000010766; -; .
HOGENOMHBG686708; -; .
ProtClustDBPRK03767; -; .
BioCycEcoCyc:PD01343; -; .
BioCycMetaCyc:PD01343; -; .
GenevestigatorP0A8G6; -; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0010181; F:FMN binding; IDA:EcoCyc; .
GOGO:0016655; F:oxidoreductase activity; acting on NADH or NADPH; quinone or similar compound as acceptor
GOGO:0045892; P:negative regulation of transcription; DNA-dependent; IEA:InterPro
GOGO:0006979; P:response to oxidative stress; IEP:EcoCyc; .
HAMAPMF_01017; Flav_WrbA; 1; -
InterProIPR008254; Flavodoxin/NO_synth; .
InterProIPR010089; Flavoprotein_WrbA; .
PfamPF00258; Flavodoxin_1; 1; .
TIGRFAMsTIGR01755; Flav_wrbA; 1; .


SWISS-2DPAGE (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server