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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A9B2




SWISS-2DPAGE:  P0A9B2


P0A9B2


General information about the entry
View entry in simple text format
Entry nameG3P1_ECOLI
Primary accession numberP0A9B2
Secondary accession number(s) P06977
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 5)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A; Short=GAPDH-A; EC=1.2.1.12;.
Gene nameName=gapA
OrderedLocusNames=b1779, JW1768
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[5]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=6.28; Mw=36820
pI=6.58; Mw=36458
pI=6.92; Mw=23143

MAPPING (identification):
SPOTS 2D-000L4P, 2D-000L4W: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2];
SPOT 2D-000LK1: MICROSEQUENCING [3].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.03; Mw=10690
pI=5.32; Mw=9173

MAPPING (identification):
MICROSEQUENCING (AAQKR AND AVGKV) [4].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.74; Mw=40430  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI6-11 {Escherichia coli(6-11)}
Escherichia coli
ECOLI6-11
  map experimental info
  protein estimated location
 
ECOLI6-11

MAP LOCATIONS:
pI=6.11; Mw=34907  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.94; Mw=36106  [identification data]
pI=5.94; Mw=22606  [identification data]

MAPPING (identification):
SPOT 2D-001WOM: Tandem mass spectrometry [5];
SPOT 2D-001WVB: Peptide mass fingerprinting [5].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0A9B2; G3P1_ECOLI.
ECO2DBASEH034.3; 6TH EDITION.
ECO2DBASEI033.5; 6TH EDITION.
UniProtKB/Swiss-ProtP0A9B2; G3P1_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameG3P1_ECOLI
Primary accession numberP0A9B2
Secondary accession number(s) P06977
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 73)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A; Short=GAPDH-A; EC=1.2.1.12;
Gene nameName=gapA
OrderedLocusNames=b1779, JW1768
Encoded onName=gapA; OrderedLocusNames=b1779, JW1768
Keywords3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX02662; CAA26498.1; -; Genomic_DNA
EMBLU00096; AAC74849.1; -; Genomic_DNA
EMBLAP009048; BAA15576.1; -; Genomic_DNA
EMBLM66870; AAA23838.1; -; Genomic_DNA
EMBLM66871; AAA23839.1; -; Genomic_DNA
EMBLM66872; AAA02930.1; -; Genomic_DNA
EMBLM66873; AAA23840.1; -; Genomic_DNA
EMBLM66874; AAA23841.1; -; Genomic_DNA
EMBLM66875; AAA23842.1; -; Genomic_DNA
EMBLM66876; AAA23843.1; -; Genomic_DNA
EMBLM66877; AAA23844.1; -; Genomic_DNA
EMBLM66878; AAA23845.1; -; Genomic_DNA
EMBLM66879; AAA23846.1; -; Genomic_DNA
EMBLM66880; AAA23847.1; -; Genomic_DNA
EMBLM66881; AAA23848.1; -; Genomic_DNA
EMBLM66882; AAA23849.1; -; Genomic_DNA
EMBLU07750; AAC43271.1; -; Genomic_DNA
EMBLU07751; AAC43272.1; -; Genomic_DNA
EMBLU07752; AAC43273.1; -; Genomic_DNA
EMBLU07754; AAC43274.1; -; Genomic_DNA
EMBLU07765; AAC43284.1; -; Genomic_DNA
EMBLU07768; AAC43285.1; -; Genomic_DNA
EMBLU07769; AAC43286.1; -; Genomic_DNA
EMBLU07770; AAC43287.1; -; Genomic_DNA
EMBLU07771; AAC43288.1; -; Genomic_DNA
EMBLU07772; AAC43289.1; -; Genomic_DNA
EMBLU07773; AAC43290.1; -; Genomic_DNA
PIRA25209; DEECG3; .
RefSeqNP_416293.1; NC_000913.2; .
PDB1DC3; X-ray; 2.50 A; A/B=2-331
PDB1DC4; X-ray; 2.50 A; A/B=2-331
PDB1DC5; X-ray; 2.00 A; A/B=2-331
PDB1DC6; X-ray; 2.00 A; A/B=2-331
PDB1GAD; X-ray; 1.80 A; O/P=2-331
PDB1GAE; X-ray; 2.17 A; O/P=2-331
PDB1S7C; X-ray; 2.04 A; A=2-330
PDB2VYN; X-ray; 2.20 A; A/B/C=1-331
PDB2VYV; X-ray; 2.38 A; A/B/C=1-331
PDBsum1DC3; -; .
PDBsum1DC4; -; .
PDBsum1DC5; -; .
PDBsum1DC6; -; .
PDBsum1GAD; -; .
PDBsum1GAE; -; .
PDBsum1S7C; -; .
PDBsum2VYN; -; .
PDBsum2VYV; -; .
ProteinModelPortalP0A9B2; -; .
SMRP0A9B2; 2-331; .
DIPDIP-31848N; -; .
IntActP0A9B2; 23; .
MINTMINT-1255410; -; .
SWISS-2DPAGEP0A9B2; -; .
2DBase-EcoliP0A9B2; -; .
ECO2DBASEH034.3; 6TH EDITION; .
ECO2DBASEI033.5; 6TH EDITION; .
PRIDEP0A9B2; -; .
EnsemblBacteriaEBESCT00000004576; EBESCP00000004576; EBESCG00000003736; .
EnsemblBacteriaEBESCT00000004577; EBESCP00000004577; EBESCG00000003736; .
EnsemblBacteriaEBESCT00000004578; EBESCP00000004578; EBESCG00000003736; .
EnsemblBacteriaEBESCT00000004579; EBESCP00000004579; EBESCG00000003736; .
EnsemblBacteriaEBESCT00000017795; EBESCP00000017086; EBESCG00000016851; .
GeneID947679; -; .
GenomeReviewsAP009048_GR; JW1768; .
GenomeReviewsU00096_GR; b1779; .
KEGGecj:JW1768; -; .
KEGGeco:b1779; -; .
EchoBASEEB0362; -; .
EcoGeneEG10367; gapA; .
eggNOGCOG0057; -; .
GeneTreeEBGT00050000009772; -; .
HOGENOMHBG571736; -; .
OMADARTSIF; -; .
ProtClustDBPRK15425; -; .
BioCycEcoCyc:GAPDH-A-MONOMER; -; .
BioCycMetaCyc:GAPDH-A-MONOMER; -; .
GenevestigatorP0A9B2; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:EC; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterProIPR020831; GlycerAld/Erythrose_P_DH; .
InterProIPR020830; GlycerAld_3-P_DH_AS; .
InterProIPR020829; GlycerAld_3-P_DH_cat; .
InterProIPR020828; GlycerAld_3-P_DH_NAD(P)-bd; .
InterProIPR006424; Glyceraldehyde-3-P_DH_1; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PANTHERPTHR10836; GAP_DH; 1; .
PfamPF02800; Gp_dh_C; 1; .
PfamPF00044; Gp_dh_N; 1; .
PIRSFPIRSF000149; GAP_DH; 1; .
PRINTSPR00078; G3PDHDRGNASE; .
SMARTSM00846; Gp_dh_N; 1; .
TIGRFAMsTIGR01534; GAPDH-I; 1; .
PROSITEPS00071; GAPDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server