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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0A9B2 |
SWISS-2DPAGE: P0A9B2
P0A9B2:
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ID G3P1_ECOLI; STANDARD; 2DG.
AC P0A9B2; P06977;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 31-MAR-2004, 2D annotation version 5.
DT 19-MAY-2011, general annotation version 13.
DE RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A; Short=GAPDH-A; EC=1.2.1.12;.
GN Name=gapA; OrderedLocusNames=b1779, JW1768;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI6-11, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI6-11, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [5]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: HOMOTETRAMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000L4P=6.28/36820;
2D -!- PI/MW: SPOT 2D-000L4W=6.58/36458;
2D -!- PI/MW: SPOT 2D-000LK1=6.92/23143; !FRAGMENT!
2D -!- MAPPING: SPOTS 2D-000L4P, 2D-000L4W: AMINO ACID COMPOSITION AND
2D MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2];
2D SPOT 2D-000LK1: MICROSEQUENCING [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0015G5=5.03/10690; !FRAGMENT!
2D -!- PI/MW: SPOT 2D-0015HJ=5.32/9173; !FRAGMENT!
2D -!- MAPPING: MICROSEQUENCING (AAQKR AND AVGKV) [4].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001LAE=5.74/40430;
2D -!- PEPTIDE MASSES: SPOT 2D-001LAE: 835.357; 894.435; 923.465;
2D 1108.633; 1161.611; 1213.652; 1401.733; 1495.839; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI6-11;
2D -!- PI/MW: SPOT 2D-001TKM=6.11/34907;
2D -!- PEPTIDE MASSES: SPOT 2D-001TKM: 923.422; 932.492; 967.428;
2D 1108.657; 1161.634; 1213.716; 1401.785; 1495.901; 1675.813; 1756.946;
2D 2590.444; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WOM=5.94/36106;
2D -!- PI/MW: SPOT 2D-001WVB=5.94/22606; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001WVB: 869.46; 923.41; 1108.57; 1161.57;
2D 1224.67; 1292.66; 1401.7; 1495.84; 1710.83; 1866.07; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WOM: (R)GASQNIIPSSTGAAK(A),198-212;
2D (K)AATYEQIK(A),249-256; (K)AGIALNDNFVK(L),296-306.
2D -!- MAPPING: SPOT 2D-001WOM: Tandem mass spectrometry [5]; SPOT
2D 2D-001WVB: Peptide mass fingerprinting [5].
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DR 2DBase-Ecoli; P0A9B2; G3P1_ECOLI.
DR ECO2DBASE; H034.3; 6TH EDITION.
DR ECO2DBASE; I033.5; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A9B2; G3P1_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4.5-5.5)
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Escherichia coli(5-6)
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Escherichia coli(6-11)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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