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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A9B2




SWISS-2DPAGE: P0A9B2



P0A9B2:

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ID   G3P1_ECOLI; STANDARD; 2DG.
AC   P0A9B2; P06977;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 13.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A; Short=GAPDH-A; EC=1.2.1.12;.
GN   Name=gapA; OrderedLocusNames=b1779, JW1768;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI6-11, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI6-11, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: HOMOTETRAMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000L4P=6.28/36820;
2D   -!-   PI/MW: SPOT 2D-000L4W=6.58/36458;
2D   -!-   PI/MW: SPOT 2D-000LK1=6.92/23143; !FRAGMENT!
2D   -!-   MAPPING: SPOTS 2D-000L4P, 2D-000L4W: AMINO ACID COMPOSITION AND
2D         MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2];
2D         SPOT 2D-000LK1: MICROSEQUENCING [3].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-0015G5=5.03/10690; !FRAGMENT!
2D   -!-   PI/MW: SPOT 2D-0015HJ=5.32/9173; !FRAGMENT!
2D   -!-   MAPPING: MICROSEQUENCING (AAQKR AND AVGKV) [4].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LAE=5.74/40430;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LAE: 835.357; 894.435; 923.465;
2D         1108.633; 1161.611; 1213.652; 1401.733; 1495.839; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI6-11;
2D   -!-   PI/MW: SPOT 2D-001TKM=6.11/34907;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001TKM: 923.422; 932.492; 967.428;
2D         1108.657; 1161.634; 1213.716; 1401.785; 1495.901; 1675.813; 1756.946;
2D         2590.444; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WOM=5.94/36106;
2D   -!-   PI/MW: SPOT 2D-001WVB=5.94/22606; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WVB: 869.46; 923.41; 1108.57; 1161.57;
2D         1224.67; 1292.66; 1401.7; 1495.84; 1710.83; 1866.07; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WOM: (R)GASQNIIPSSTGAAK(A),198-212;
2D         (K)AATYEQIK(A),249-256; (K)AGIALNDNFVK(L),296-306.
2D   -!-   MAPPING: SPOT 2D-001WOM: Tandem mass spectrometry [5]; SPOT
2D         2D-001WVB: Peptide mass fingerprinting [5].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   2DBase-Ecoli; P0A9B2; G3P1_ECOLI.
DR   ECO2DBASE; H034.3; 6TH EDITION.
DR   ECO2DBASE; I033.5; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A9B2; G3P1_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli(6-11)
ECOLI6-11
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
 


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server