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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0A9P0 |
SWISS-2DPAGE: P0A9P0
P0A9P0:
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ID DLDH_ECOLI; STANDARD; 2DG.
AC P0A9P0; P00391;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 31-MAR-2004, 2D annotation version 2.
DT 19-MAY-2011, general annotation version 14.
DE RecName: Full=Dihydrolipoyl dehydrogenase; EC=1.8.1.4; AltName:
DE Full=Dihydrolipoamide dehydrogenase; AltName: Full=E3 component of
DE pyruvate and 2-oxoglutarate dehydrogenases complexes; AltName:
DE Full=Glycine cleavage system L protein;.
GN Name=lpdA; Synonyms=lpd; OrderedLocusNames=b0116, JW0112;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP PROTEIN EXPRESSION.
RX MEDLINE=87194592; PubMed=3553157; [NCBI, Expasy, EBI, Israel, Japan]
RA Jones P.G., Vanbogelen R.A., Neidhardt F.C.;
RT ''''''Induction of proteins in response to low temperature in Escherichia coli'';'';'';
RL J. Bacteriol. 169(1):2092-2095(1987).
RN [4]
RP PROTEIN EXPRESSION.
RX MEDLINE=83160794; PubMed=6339477; [NCBI, Expasy, EBI, Israel, Japan]
RA Smith M.W., Neidhardt F.C.;
RT ''''''Proteins induced by aerobiosis in Escherichia coli'';'';'';
RL J. Bacteriol. 154(1):344-350(1983).
RN [5]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: HOMODIMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KPM=5.81/53704;
2D -!- EXPRESSION: INCREASED DURING AEROBIC GROWTH [4]; INCREASED DURING
2D THE ADJUSTMENT OF E. COLI TO GROWTH AT 10°C [3].
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WIU=5.82/50800;
2D -!- PI/MW: SPOT 2D-001WS6=5.90/28059; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001WIU: 1011.49; 1076.62; 1139.62; 1144.64;
2D 1257.61; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WS6: 860.4; 896.44; 934.42; 1076.56;
2D 1144.56; 1333.65; 1473.76; 1571.82; 1601.86; 1713.83; 1825.98; 1971.01;
2D 2202.14; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WS6: (K)APAEPQRYDAVLVAIGR(V),256-272;
2D (R)YDAVLVAIGR(V),263-272; (K)AGVEVDDRGFIR(V),284-295; (K)VIPSIAYTEPEVAWVGLTEK(E),345-364.
2D -!- MAPPING: Peptide mass fingerprinting [5]; SPOT 2D-001WS6: TANDEM
2D MASS SPECTROMETRY [5].
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DR 2DBase-Ecoli; P0A9P0; DLDH_ECOLI.
DR ECO2DBASE; G050.5; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A9P0; DLDH_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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