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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0ABA0 |
SWISS-2DPAGE: P0ABA0
P0ABA0:
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ID ATPF_ECOLI; STANDARD; 2DG.
AC P0ABA0; P00859;
DT 15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT 31-MAR-2004, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 7.
DE RecName: Full=ATP synthase subunit b; AltName: Full=ATP synthase F(0)
DE sector subunit b; AltName: Full=ATPase subunit I; AltName: Full=F-type
DE ATPase subunit b; Short=F-ATPase subunit b;.
GN Name=atpF; Synonyms=papF, uncF; OrderedLocusNames=b3736, JW3714;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI-DIGE4.5-6.5.
IM ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: F-TYPE ATPASES HAVE 2 COMPONENTS, CF(1) - THE CATALYTIC CORE
CC - AND CF(0) - THE MEMBRANE PROTON CHANNEL. CF(1) HAS FIVE SUBUNITS:
CC ALPHA(3), BETA(3), GAMMA(1), DELTA(1), EPSILON(1). CF(0) HAS THREE MAIN
CC SUBUNITS: A, B AND C
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WWG=5.53/19289;
2D -!- PI/MW: SPOT 2D-001WWO=5.53/18722;
2D -!- PEPTIDE MASSES: SPOT 2D-001WWG: 1059.57; 1131.53; 1169.63; 1313.66;
2D 1387.71; 1427.74; 1469.79; 1512.8; 1555.83; 1716.84; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WWO: 961.46; 1059.56; 1169.63; 1297.74;
2D 1313.66; 1387.71; 1427.75; 1469.78; 1512.82; 1555.84; 1656.9; 1680.89;
2D 1716.84; 1887.9; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WWO: (R)QKEIADGLASAER(A),37-49;
2D (K)EIADGLASAER(A),39-49; (K)ASATDQLKK(A),59-67; (K)AEAQVIIEQANK(R),70-81;
2D (R)SQILDEAK(A),84-91; (R)SQILDEAKAEAEQER(T),84-98; (K)IVAQAQAEIEAER(K),101-113.
2D -!- EXPRESSION: decrease after benzoic acid treatment [1].
2D -!- MAPPING: Peptide mass fingerprinting [1]; SPOT 2D-001WWO: TANDEM
2D MASS SPECTROMETRY [1].
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CC This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
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DR UniProtKB/Swiss-Prot; P0ABA0; ATPF_ECOLI.
//
The following 2-D map is available for this protein:
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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