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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0ABK5




SWISS-2DPAGE:  P0ABK5


P0ABK5


General information about the entry
View entry in simple text format
Entry nameCYSK_ECOLI
Primary accession numberP0ABK5
Secondary accession number(s) P11096
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 5)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Cysteine synthase A; Short=CSase A; EC=2.5.1.47; AltName: Full=O-acetylserine (thiol)-lyase A; Short=OAS-TL A; AltName: Full=O-acetylserine sulfhydrylase A; AltName: Full=Sulfate starvation-induced protein 5; Short=SSI5;.
Gene nameName=cysK
Synonyms=cysZ
OrderedLocusNames=b2414, JW2407
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[4]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.80; Mw=36099

MAPPING (identification):
AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI4-5 {Escherichia coli(4-5)}
Escherichia coli
ECOLI4-5
  map experimental info
  protein estimated location
 
ECOLI4-5

MAP LOCATIONS:
pI=5.01; Mw=26299  [identification data]
pI=4.95; Mw=25617  [identification data]
pI=4.95; Mw=24067  [identification data]
pI=4.93; Mw=22242  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [3];
SPOT 2D-001HLU: MICROSEQUENCING (XLGA) [3].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.32; Mw=32863  [identification data]
pI=5.06; Mw=29467  [identification data]
pI=5.04; Mw=28314  [identification data]
pI=4.95; Mw=28177  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [3];
SPOT 2D-00158P: MICROSEQUENCING (XYKL) [3].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.78; Mw=42978  [identification data]
pI=5.10; Mw=41867  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [3].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.83; Mw=34342  [identification data]

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [4].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0ABK5; CYSK_ECOLI.
UniProtKB/Swiss-ProtP0ABK5; CYSK_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCYSK_ECOLI
Primary accession numberP0ABK5
Secondary accession number(s) P11096 P21546
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 56)
Name and origin of the protein
DescriptionRecName: Full=Cysteine synthase A; Short=CSase A; EC=2.5.1.47; AltName: Full=O-acetylserine (thiol)-lyase A; Short=OAS-TL A; AltName: Full=O-acetylserine sulfhydrylase A; AltName: Full=Sulfate starvation-induced protein 5; Short=SSI5;
Gene nameName=cysK
Synonyms=cysZ
OrderedLocusNames=b2414, JW2407
Encoded onName=cysK; Synonyms=cysZ; OrderedLocusNames=b2414, JW2407
KeywordsAllosteric enzyme; Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Direct protein sequencing; Pyridoxal phosphate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX12615; CAA31137.1; -; Genomic_DNA
EMBLM21451; AAA23654.1; -; Genomic_DNA
EMBLU00096; AAC75467.1; -; Genomic_DNA
EMBLAP009048; BAA16288.1; -; Genomic_DNA
EMBLM21994; AAA24383.1; -; Genomic_DNA
PIRE65015; SYECAC; .
RefSeqNP_416909.1; NC_000913.2; .
ProteinModelPortalP0ABK5; -; .
SMRP0ABK5; 2-305; .
DIPDIP-36170N; -; .
IntActP0ABK5; 13; .
MINTMINT-1240013; -; .
SWISS-2DPAGEP0ABK5; -; .
2DBase-EcoliP0ABK5; -; .
EnsemblBacteriaEBESCT00000003304; EBESCP00000003304; EBESCG00000002708; .
EnsemblBacteriaEBESCT00000015969; EBESCP00000015260; EBESCG00000015029; .
GeneID946877; -; .
GenomeReviewsAP009048_GR; JW2407; .
GenomeReviewsU00096_GR; b2414; .
KEGGecj:JW2407; -; .
KEGGeco:b2414; -; .
EchoBASEEB0189; -; .
EcoGeneEG10192; cysK; .
eggNOGCOG0031; -; .
GeneTreeEBGT00050000009701; -; .
HOGENOMHBG748215; -; .
OMAAHSIGNT; -; .
ProtClustDBPRK10717; -; .
BioCycEcoCyc:ACSERLYA-MONOMER; -; .
BioCycMetaCyc:ACSERLYA-MONOMER; -; .
GenevestigatorP0ABK5; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0004124; F:cysteine synthase activity; IDA:EcoCyc; .
GOGO:0080146; F:L-cysteine desulfhydrase activity; IMP:EcoCyc; .
GOGO:0030170; F:pyridoxal phosphate binding; IEA:InterPro; .
GOGO:0016740; F:transferase activity; IEA:UniProtKB-KW; .
GOGO:0006535; P:cysteine biosynthetic process from serine; IDA:EcoCyc; .
InterProIPR001216; Cys_synth_BS; .
InterProIPR005856; Cys_synthKM; .
InterProIPR005859; CysK; .
InterProIPR001926; PyrdxlP-dep_enz_bsu; .
PfamPF00291; PALP; 1; .
SUPFAMSSF53686; PyrdxlP-dep_enz_bsu; 1; .
TIGRFAMsTIGR01139; CysK; 1; .
TIGRFAMsTIGR01136; CysKM; 1; .
PROSITEPS00901; CYS_SYNTHASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server