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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0ADG7 |
SWISS-2DPAGE: P0ADG7
P0ADG7:
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ID IMDH_ECOLI; STANDARD; 2DG.
AC P0ADG7; P06981;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 31-MAR-2004, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 13.
DE RecName: Full=Inosine-5'-monophosphate dehydrogenase; Short=IMP
DE dehydrogenase; Short=IMPD; Short=IMPDH; EC=1.1.1.205;.
GN Name=guaB; Synonyms=guaR; OrderedLocusNames=b2508, JW5401;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [5]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: HOMOTETRAMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KN3=5.76/56743;
2D -!- PI/MW: SPOT 2D-000KOE=6.01/55094;
2D -!- MAPPING: SPOT 2D-000KN3: AMINO ACID COMPOSITION AND SEQUENCE TAG
2D (MLRI) [3]; SPOT 2D-000KOE: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON
2D 2-D GELS BY VANBOGELEN [2].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001LC6=5.49/38144; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001LC6: 1217.688; 1257.669; 1302.672;
2D 1786.983; 1855.026; 1986.145; 2179.223; 2335.299; 2757.502; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WH8=5.99/55298;
2D -!- PEPTIDE MASSES: SPOT 2D-001WH8: 850.49; 887.43; 969.5; 1076.48;
2D 1158.48; 1217.6; 1257.58; 1302.61; 1637.93; 1786.92; 1891.99; 1986.09;
2D 2109.03; 2179.16; 2235.19; 2757.45; 3209.68; 3258.79; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WH8:
2D (R)NGFAGYPVVTEENELVGIITGR(D),116-137; (R)IVTGVGVPQITAVADAVEALEGTGIPVIADGGIR(F),309-342.
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [5].
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DR UniProtKB/Swiss-Prot; P0ADG7; IMDH_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
 |
Escherichia coli(5-6)
 |
Escherichia coli DIGE (4.5-6.5)
 |
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SWISS-2DPAGE (search AC)
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