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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AEM9




SWISS-2DPAGE: P0AEM9



P0AEM9:

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ID   FLIY_ECOLI; STANDARD; 2DG.
AC   P0AEM9; P39174;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   15-MAY-2003, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 13.
DE   RecName: Full=Cystine-binding periplasmic protein; Short=CBP; AltName:
DE   Full=Protein fliY; AltName: Full=Sulfate starvation-induced protein 7;
DE   Short=SSI7; Flags: Precursor;.
GN   Name=fliY; Synonyms=yzzR; OrderedLocusNames=b1920, JW1905;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000LFY=5.01/26225;
2D   -!-   PI/MW: SPOT 2D-000LGC=5.11/25827;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000LGC: B=12, Z=11.5, S=5.2, H=0.4,
2D         G=10.9, T=6, A=7.9, P=2.4, Y=2.1, R=4.3, V=8.2, M=3.7, I=4.3, L=9.9,
2D         F=3.3, K=8;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (DEGL) [1].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-00159C=5.23/28658;
2D   -!-   PI/MW: SPOT 2D-0015AN=5.19/26525;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0015AN: 914.062; 1014.04; 1481.21; 1592.28;
2D         1600.3; 1756.44; TRYPSIN.
2D   -!-   MAPPING: SPOT 2D-00159C:  MICROSEQUENCING (DEGL) [2]; SPOT
2D         2D-0015AN: Peptide mass fingerprinting [2].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WTE=5.17/25686;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WTE: 1014.52; 1061.54; 1205.58; 1481.73;
2D         1580.8; 1600.84; 1756.92; 1902.95; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [3].
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DR   2DBase-Ecoli; P0AEM9; FLIY_ECOLI.
DR   UniProtKB/Swiss-Prot; P0AEM9; FLIY_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server