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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AEU0




SWISS-2DPAGE: P0AEU0



P0AEU0:

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ID   HISJ_ECOLI; STANDARD; 2DG.
AC   P0AEU0; P39182;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   15-MAY-2003, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=Histidine-binding periplasmic protein; Short=HBP; Flags: Precursor;.
GN   Name=hisJ; OrderedLocusNames=b2309, JW2306;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000LDL=5.05/28670;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (AIPQ) [1].
2D   -!- MASTER: ECOLI4-5;
2D   -!-   PI/MW: SPOT 2D-001HPA=4.99/10511; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HPA: 903.396; 1107.575; 1123.554;
2D         1210.491; 1810.911; 2199.188; 2299.245; 2355.312; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-00159H=5.14/28451;
2D   -!-   PEPTIDE MASSES: SPOT 2D-00159H: 903.331; 1123.44; 1210.39; 1235.43;
2D         1407.41; 1425.5; 1605.6; 1691.55; 1707.58; 1810.63; 2298.8; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2], and microsequencing
2D         (AIPQ) [2].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WS8=5.28/27969;
2D   -!-   PI/MW: SPOT 2D-001WSD=5.14/27701;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WS8: 1079.54; 1123.64; 1235.71; 1330.76;
2D         1425.8; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WSD: 1079.5; 1123.58; 1235.67; 1330.67;
2D         1504.67; 1551.7; 1605.77; 1810.87; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [3].
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CC   its content is in no way modified and this statement is not removed.
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DR   2DBase-Ecoli; P0AEU0; HISJ_ECOLI.
DR   UniProtKB/Swiss-Prot; P0AEU0; HISJ_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4-5)
ECOLI4-5
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
  


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server