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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AF12




SWISS-2DPAGE:  P0AF12


P0AF12


General information about the entry
View entry in simple text format
Entry nameMTNN_ECOLI
Primary accession numberP0AF12
Secondary accession number(s) P24247
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 17)
Name and origin of the protein
DescriptionRecName: Full=5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase; Short=MTA/SAH nucleosidase; Short=MTAN; EC=3.2.2.9; AltName: Full=5'-methylthioadenosine nucleosidase; Short=MTA nucleosidase; AltName: Full=P46; AltName: Full=S-adenosylhomocysteine nucleosidase; Short=AdoHcy nucleosidase; Short=SAH nucleosidase; Short=SRH nucleosidase;.
Gene nameName=mtnN
Synonyms=mtn, pfs, yadA
OrderedLocusNames=b0159, JW0155
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.06; Mw=25959

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEC025.4; 6TH EDITION.
UniProtKB/Swiss-ProtP0AF12; MTNN_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameMTNN_ECOLI
Primary accession numberP0AF12
Secondary accession number(s) P24247
Sequence was last modified on December 20, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 60)
Name and origin of the protein
DescriptionRecName: Full=5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase; Short=MTA/SAH nucleosidase; Short=MTAN; EC=3.2.2.9; AltName: Full=5'-methylthioadenosine nucleosidase; Short=MTA nucleosidase; AltName: Full=P46; AltName: Full=S-adenosylhomocysteine nucleosidase; Short=AdoHcy nucleosidase; Short=SAH nucleosidase; Short=SRH nucleosidase;
Gene nameName=mtnN
Synonyms=mtn, pfs, yadA
OrderedLocusNames=b0159, JW0155
Encoded onName=mtnN; Synonyms=mtn, pfs, yadA; OrderedLocusNames=b0159, JW0155
Keywords3D-structure; Amino-acid biosynthesis; Complete proteome; Hydrolase; Methionine biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM31772; AAA23678.1; ALT_INIT; Genomic_DNA
EMBLU24438; AAC38291.1; -; Genomic_DNA
EMBLM83735; AAA24322.1; -; Genomic_DNA
EMBLU70214; AAB08589.1; -; Genomic_DNA
EMBLU00096; AAC73270.1; -; Genomic_DNA
EMBLAP009048; BAB96736.1; -; Genomic_DNA
PIRS45227; S45227; .
RefSeqNP_414701.1; NC_000913.2; .
PDB1JYS; X-ray; 1.90 A; A/B=1-232
PDB1NC1; X-ray; 2.00 A; A/B=1-232
PDB1NC3; X-ray; 2.20 A; A/B=1-232
PDB1Y6Q; X-ray; 2.20 A; A/B=1-232
PDB1Y6R; X-ray; 2.20 A; A/B=1-232
PDB1Z5N; X-ray; 2.10 A; A/B=1-232
PDB1Z5O; X-ray; 2.00 A; A/B=1-232
PDB1Z5P; X-ray; 2.00 A; A=1-232
PDB3O4V; X-ray; 1.75 A; A/B=1-232
PDBsum1JYS; -; .
PDBsum1NC1; -; .
PDBsum1NC3; -; .
PDBsum1Y6Q; -; .
PDBsum1Y6R; -; .
PDBsum1Z5N; -; .
PDBsum1Z5O; -; .
PDBsum1Z5P; -; .
PDBsum3O4V; -; .
ProteinModelPortalP0AF12; -; .
SMRP0AF12; 1-232; .
IntActP0AF12; 6; .
SWISS-2DPAGEP0AF12; -; .
ECO2DBASEC025.4; 6TH EDITION; .
EnsemblBacteriaEBESCT00000000541; EBESCP00000000541; EBESCG00000000455; .
EnsemblBacteriaEBESCT00000015764; EBESCP00000015055; EBESCG00000014824; .
GeneID948542; -; .
GenomeReviewsAP009048_GR; JW0155; .
GenomeReviewsU00096_GR; b0159; .
KEGGecj:JW0155; -; .
KEGGeco:b0159; -; .
EchoBASEEB1082; -; .
EcoGeneEG11090; pfs; .
eggNOGCOG0775; -; .
GeneTreeEBGT00050000011162; -; .
HOGENOMHBG367723; -; .
OMAVIGAMEQ; -; .
ProtClustDBPRK05584; -; .
BioCycEcoCyc:EG11090-MONOMER; -; .
BioCycMetaCyc:EG11090-MONOMER; -; .
DrugBankDB00173; Adenine; .
GenevestigatorP0AF12; -; .
GOGO:0008782; F:adenosylhomocysteine nucleosidase activity; IDA:EcoCyc; .
GOGO:0008930; F:methylthioadenosine nucleosidase activity; IDA:EcoCyc; .
GOGO:0003001; P:generation of a signal involved in cell-cell signaling; IDA:EcoCyc; .
GOGO:0019284; P:L-methionine biosynthetic process from S-adenosylmethionine; IDA:EcoCyc; .
GOGO:0019509; P:L-methionine salvage from methylthioadenosine; IEA:InterPro; .
GOGO:0009164; P:nucleoside catabolic process; IEA:InterPro; .
HAMAPMF_01684; Salvage_tnN; 1; -
InterProIPR010049; MTA_SAH_Nsdase; .
InterProIPR018017; Nucleoside_phosphorylase; .
InterProIPR000845; Nucleoside_phosphorylase_d; .
PANTHERPTHR21234; PNP_UDP; 1; .
PANTHERPTHR21234:SF6; PTHR21234:SF6; 1; .
PfamPF01048; PNP_UDP_1; 1; .
TIGRFAMsTIGR01704; MTA/SAH-Nsdase; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server