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Sample Preparation and Post-separation Analysis

Searching in 'SWISS-2DPAGE' for entry matching: P0AFC7



General information about the entry
View entry in simple text format
Entry nameNUOB_ECOLI
Primary accession numberP0AFC7
Secondary accession number(s) P33598
integrated into SWISS-2DPAGE on April 1, 2000 (release 12)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 9)
Name and origin of the protein
DescriptionRecName: Full=NADH-quinone oxidoreductase subunit B; EC=; AltName: Full=NADH dehydrogenase I subunit B; AltName: Full=NDH-1 subunit B; AltName: Full=NUO2;.
Gene nameName=nuoB
OrderedLocusNames=b2287, JW5875
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
  map experimental info
  protein estimated location

pI=5.42; Mw=30843  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
  map experimental info
  protein estimated location

pI=5.46; Mw=25413  [identification data]

MAPPING (identification):
Tandem mass spectrometry [2].

This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See or send email from
UniProtKB/Swiss-ProtP0AFC7; NUOB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameNUOB_ECOLI
Primary accession numberP0AFC7
Secondary accession number(s) P33598 P78090 P78186 P78187
Sequence was last modified on December 20, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 52)
Name and origin of the protein
DescriptionRecName: Full=NADH-quinone oxidoreductase subunit B; EC=; AltName: Full=NADH dehydrogenase I subunit B; AltName: Full=NDH-1 subunit B; AltName: Full=NUO2;
Gene nameName=nuoB
OrderedLocusNames=b2287, JW5875
Encoded onName=nuoB; OrderedLocusNames=b2287, JW5875
Keywords4Fe-4S; Cell inner membrane; Cell membrane; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Membrane; Metal-binding; NAD; Oxidoreductase; Quinone; Reference proteome; Transport; Ubiquinone.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLX68301; CAA48361.1; -; Genomic_DNA
EMBLU00096; AAC75347.1; -; Genomic_DNA
EMBLAP009048; BAA16121.2; -; Genomic_DNA
PIRE65000; E65000; .
RefSeqNP_416790.1; NC_000913.2; .
ProteinModelPortalP0AFC7; -; .
SMRP0AFC7; 34-181; .
IntActP0AFC7; 1; .
TCDB3.D.1.1.1; H+ or Na+-translocating NADH dehydrogenase (NDH) family; .
EnsemblBacteriaEBESCT00000002390; EBESCP00000002390; EBESCG00000001951; .
EnsemblBacteriaEBESCT00000002391; EBESCP00000002391; EBESCG00000001951; .
EnsemblBacteriaEBESCT00000016093; EBESCP00000015384; EBESCG00000015153; .
GeneID946738; -; .
GenomeReviewsAP009048_GR; JW5875; .
GenomeReviewsU00096_GR; b2287; .
KEGGecj:JW5875; -; .
KEGGeco:b2287; -; .
EchoBASEEB2008; -; .
EcoGeneEG12083; nuoB; .
eggNOGCOG0377; -; .
GeneTreeEBGT00050000009829; -; .
HOGENOMHBG553221; -; .
ProtClustDBPRK06411; -; .
BioCycEcoCyc:NUOB-MONOMER; -; .
BioCycMetaCyc:NUOB-MONOMER; -; .
GenevestigatorP0AFC7; -; .
GOGO:0045272; C:plasma membrane respiratory chain complex I; IDA:EcoCyc; .
GOGO:0051539; F:4 iron; 4 sulfur cluster binding; IMP:EcoCyc
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IMP:EcoCyc; .
GOGO:0015453; F:oxidoreduction-driven active transmembrane transporter activity; IDA:EcoliWiki; .
GOGO:0048038; F:quinone binding; IEA:UniProtKB-KW; .
GOGO:0009060; P:aerobic respiration; IMP:EcoCyc; .
GOGO:0009061; P:anaerobic respiration; IMP:EcoliWiki; .
GOGO:0015990; P:electron transport coupled proton transport; IMP:EcoCyc; .
HAMAPMF_01356; NDH1_NuoB; 1; -
InterProIPR006137; NADH_UbQ_OxRdtase-like_20kDa; .
InterProIPR006138; NADH_UQ_OxRdtase_20Kd_su; .
InterProIPR014406; NiFe-hyd_3_ssu/Q_oxred_NuoB; .
Gene3DG3DSA:; G3DSA:; 1; .
PANTHERPTHR11995; NiFe_hyd_3_ssu/Q_oxred_NuoB; 1; .
PfamPF01058; Oxidored_q6; 1; .
TIGRFAMsTIGR01957; NuoB_fam; 1; .
PROSITEPS01150; COMPLEX1_20K; 1; .


SWISS-2DPAGE (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server