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Sample Preparation and Post-separation Analysis

P0AFG0:

ID   NUSG_ECOLI; STANDARD; 2DG.
AC   P0AFG0; P16921;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 9.
DE   RecName: Full=Transcription antitermination protein nusG;.
GN   Name=nusG; OrderedLocusNames=b3982, JW3945;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6, ECOLI6-11.
IM   ECOLI, ECOLI5-6, ECOLI6-11.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000LKQ=5.93/22195;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (SEAP) [1].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LSR=5.44/14684;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LSR: 864.74; 1105.967; 1178.906; 1194.9;
2D         1266.076; 1463.123; 1534.173; 1848.43; 1864.397; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: ECOLI6-11;
2D   -!-   PI/MW: SPOT 2D-001TRH=6.40/21860;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001TRH: 1105.727; 1178.655; 1462.807;
2D         1545.821; 2243.123; 2618.295; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
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DR   UniProtKB/Swiss-Prot; P0AFG0; NUSG_ECOLI.
//

The following 2-D maps are available for this protein:

	Escherichia coli	Escherichia coli(5-6)	Escherichia coli(6-11)

SWISS-2DPAGE (search AC)