| General information about the entry |
| View entry in simple text format |
| Entry name | EFTU2_ECOLI |
| Primary accession number | P0CE48 |
| Secondary accession number(s) | P02990 P0A6N1 |
| integrated into SWISS-2DPAGE on | August 1, 1995 (release 2) |
| 2D Annotations were last modified on | May 15, 2003 (version 5) |
| General Annotations were last modified on | May 19, 2011 (version 12) |
| Name and origin of the protein |
| Description | RecName: Full=Elongation factor Tu 2; Short=EF-Tu 2; AltName: Full=P-43;. |
| Gene name | Name=tufB
OrderedLocusNames=b3980, JW3943
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| Annotated species | Escherichia coli [TaxID: 562] |
| Taxonomy | Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. |
| References |
| [1] |
MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
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| [2] |
MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
|
| [3] |
MAPPING ON GEL
MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
Bloch P.L., Phillips T.A., Neidhardt F.C.
''''''Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins'';'';''
J. Bacteriol. 141(1):1409-1420(1980)
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| [4] |
PROTEIN EXPRESSION
MEDLINE=79216190; PubMed=156716; [NCBI, Expasy, EBI, Israel, Japan]
Herendeen S.L., Vanbogelen R.A., Neidhardt F.C.
''''''Levels of major proteins of Escherichia coli during growth at different temperatures'';'';''
J. Bacteriol. 139(1):185-194(1979)
|
| [5] |
PROTEIN EXPRESSION
MEDLINE=78212912; PubMed=352533; [NCBI, Expasy, EBI, Israel, Japan]
Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.
''''''Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates'';'';''
Cell 14(1):179-190(1978)
|
| [6] |
MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
|
| [7] |
MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
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| Comments |
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| 2D PAGE maps for identified proteins
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How to interpret a protein
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ECOLI {Escherichia coli}
Escherichia coli
map experimental info
protein estimated location
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ECOLI
MAP LOCATIONS:
EXPRESSION:
CHANGED VERY LITTLE THROUGHOUT THE NORMAL ARRHENIUS TEMPERATURE RANGE (23 TO 37°C) [4]; INCREASED IN LEVEL WITH INCREASING GROWTH RATE [5].
MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3].
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ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
map experimental info
protein estimated location
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ECOLI4.5-5.5
MAP LOCATIONS:
MAPPING (identification):
Peptide mass fingerprinting [6].
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ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
map experimental info
protein estimated location
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ECOLI5-6
MAP LOCATIONS:
MAPPING (identification):
Peptide mass fingerprinting [6].
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ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
map experimental info
protein estimated location
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ECOLI-DIGE4.5-6.5
MAP LOCATIONS:
EXPRESSION:
increase after benzoic acid treatment [7].
MAPPING (identification):
| SPOTS 1WL*: Peptide mass fingerprinting [7]; SPOTS 1WQY, 1WTU, 1WLU: Tandem mass spectrometry [7]. |
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| Copyright |
| This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). |
| Cross-references |
| 2DBase-Ecoli | P0A6N1; EFTU_ECOLI. |
| ECO2DBASE | E042.0; 6TH EDITION. |
| UniProtKB/Swiss-Prot | P0CE48; EFTU2_ECOLI. |
| 2D PAGE maps for identified proteins
|
- How to interpret a protein map
- You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
- Warning 1: the displayed region reflects
an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information.
It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
- Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
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