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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0CE48




SWISS-2DPAGE: P0CE48



P0CE48:

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ID   EFTU2_ECOLI; STANDARD; 2DG.
AC   P0CE48; P02990; P0A6N1;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   15-MAY-2003, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=Elongation factor Tu 2; Short=EF-Tu 2; AltName: Full=P-43;.
GN   Name=tufB; OrderedLocusNames=b3980, JW3943;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT   ''''''Protein identifications of O'Farrell two-dimensional gels:
RT   locations of 81 Escherichia coli proteins'';'';'';
RL   J. Bacteriol. 141(1):1409-1420(1980).
RN   [4]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=79216190; PubMed=156716; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Herendeen S.L., Vanbogelen R.A., Neidhardt F.C.;
RT   ''''''Levels of major proteins of Escherichia coli during growth at
RT   different temperatures'';'';'';
RL   J. Bacteriol. 139(1):185-194(1979).
RN   [5]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=78212912; PubMed=352533; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.;
RT   ''''''Patterns of protein synthesis in E. coli: a catalog of the amount
RT   of 140 individual proteins at different growth rates'';'';'';
RL   Cell 14(1):179-190(1978).
RN   [6]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [7]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: MONOMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KWF=5.32/44704;
2D   -!-   EXPRESSION: CHANGED VERY LITTLE THROUGHOUT THE NORMAL ARRHENIUS
2D         TEMPERATURE RANGE (23 TO 37C) [4]; INCREASED IN LEVEL WITH INCREASING
2D         GROWTH RATE [5].
2D   -!-   MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D         [2] AND BLOCH [3].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-0014Y6=5.58/45311;
2D   -!-   PI/MW: SPOT 2D-00158F=5.14/30129; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-0014Y6: 1027.808; 1203.789; 1214.911;
2D         1233.878; 1390.973; 1490.167; 1618.137; 1796.385; 1804.285; 1813.378;
2D         1962.479; 1965.397; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-00158F: 1171.256; 1203.135; 1218.182;
2D         1303.349; 1390.194; 1489.424; 1768.237; 2167.337; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [6].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001L13=5.39/51249;
2D   -!-   PI/MW: SPOT 2D-001L1Y=5.20/51059;
2D   -!-   PI/MW: SPOT 2D-001L2D=5.32/50990;
2D   -!-   PI/MW: SPOT 2D-001L2E=5.35/50921;
2D   -!-   PI/MW: SPOT 2D-001L6H=5.34/50034;
2D   -!-   PI/MW: SPOT 2D-001L7G=5.31/48144;
2D   -!-   PI/MW: SPOT 2D-001L7Q=5.33/47448;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L13: 1027.686; 1233.741; 1768.93;
2D         1781.083; 1796.085; 1804.033; 1962.177; 1965.13; 2117.35; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L1Y: 1027.599; 1233.617; 1796.913;
2D         1962.031; 1964.982; 2117.176; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L2D: 1027.699; 1171.772; 1203.675;
2D         1218.707; 1233.768; 1689.98; 1768.981; 1796.163; 1804.075; 1962.231;
2D         1965.178; 2117.363; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L2E: 1027.725; 1130.675; 1171.788;
2D         1203.668; 1233.786; 1376.81; 1490.054; 1575.135; 1689.945; 1769.026;
2D         1781.199; 1796.181; 1804.163; 1813.118; 1962.272; 2117.446; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L6H: 1027.694; 1171.76; 1203.627;
2D         1214.737; 1218.674; 1376.782; 1489.988; 1575.116; 1768.959; 1796.115;
2D         1804.059; 1962.214; 1965.152; 2117.375; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L7G: 1027.405; 1489.751; 1803.839;
2D         1962.019; 1964.948; 2117.178; 2729.45; 2745.395; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L7Q: 1027.705; 1171.768; 1203.657;
2D         1214.745; 1768.965; 1796.141; 1804.088; 1962.244; 1965.174; 2117.39; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [6].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WLP=5.38/41860;
2D   -!-   PI/MW: SPOT 2D-001WLU=5.40/42307;
2D   -!-   PI/MW: SPOT 2D-001WQY=5.07/32442; !FRAGMENT!
2D   -!-   PI/MW: SPOT 2D-001WTU=5.69/25098; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WLP: 1171.69; 1187.58; 1214.69; 1233.6;
2D         1376.67; 1797.02; 2117.36; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WLU: 1027.55; 1073.54; 1171.57; 1218.55;
2D         1233.56; 1290.57; 1303.7; 1331.57; 1376.58; 1489.82; 1603.77; 1657.87;
2D         1710.92; 1728.88; 1768.82; 1795.9; 1803.86; 1812.9; 1962.04; 1964.99;
2D         2117.16; 2174.2; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WLU: (R)QVGVPYIIVFLNK(C),124-136; (K)ILELAGFLDSYIPEPER(A),188-204.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WQY: (R)AIDKPFLLPIEDVFSISGR(G),205-223.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WTU:
2D         (R)AIDKPFLLPIEDVFSISGR(G),205-223; (R)GTVVTGRVER(G),224-233;
2D         (K)VGEEVEIVGIKETQK(S),238-252; (K)LLDEGRAGENVGVLLR(G),264-279; (R)AGENVGVLLR(G),270-279.
2D   -!-   EXPRESSION: increase after benzoic acid treatment [7].
2D   -!-   MAPPING: SPOTS 1WL*: Peptide mass fingerprinting [7]; SPOTS 1WQY,
2D         1WTU, 1WLU: Tandem mass spectrometry [7].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
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DR   2DBase-Ecoli; P0A6N1; EFTU_ECOLI.
DR   ECO2DBASE; E042.0; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0CE48; EFTU2_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
  


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server